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To this end<jats:italic>B. pertussis<\/jats:italic>Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence. The expression and assembly of the fusion protein into pentameric structures (CTB-Fim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50\u2009<jats:italic>\u03bc<\/jats:italic>g of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>or<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><mml:mi>,<\/mml:mi><\/mml:math>resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1\/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against<jats:italic>Bordetella pertussis<\/jats:italic>infection.<\/jats:p>","DOI":"10.1155\/2014\/421486","type":"journal-article","created":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T21:15:20Z","timestamp":1400015720000},"page":"1-11","source":"Crossref","is-referenced-by-count":12,"title":["Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against<i>Bordetella pertussis<\/i>Infection"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9492-6485","authenticated-orcid":true,"given":"Noelia","family":"Olivera","sequence":"first","affiliation":[{"name":"Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celina E.","family":"Castuma","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Hozbor","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda E.","family":"Gaillard","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda y Biolog\u00eda Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-La Plata, CONICET-UNLP, Calle 49 y 115, 1900 La Plata, 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