{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:33:40Z","timestamp":1769603620664,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Key Basic Research Project of Shanghai Science and Technology Commission","award":["11JC1401800"],"award-info":[{"award-number":["11JC1401800"]}]},{"name":"Key Basic Research Project of Shanghai Science and Technology Commission","award":["XBR2011044"],"award-info":[{"award-number":["XBR2011044"]}]},{"name":"Shanghai Municipal Health Bureau Hundred Talents Program","award":["11JC1401800"],"award-info":[{"award-number":["11JC1401800"]}]},{"name":"Shanghai Municipal Health Bureau Hundred Talents Program","award":["XBR2011044"],"award-info":[{"award-number":["XBR2011044"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>A multiplex snapback primer system was developed for the simultaneous detection of<jats:italic>JAK2<\/jats:italic>V617F and<jats:italic>MPL<\/jats:italic>W515L\/K mutations in Philadelphia chromosome- (Ph-) negative myeloproliferative neoplasms (MPNs). The multiplex system comprises two snapback versus limiting primer sets for<jats:italic>JAK2<\/jats:italic>and<jats:italic>MPL<\/jats:italic>mutation enrichment and detection, respectively. Linear-After exponential (LATE) PCR strategy was employed for the primer design to maximize the amplification efficiency of the system. Low ionic strength buffer and rapid PCR protocol allowed for selective amplification of the mutant alleles. Amplification products were analyzed by melting curve analysis for mutation identification. The multiplex system archived 0.1% mutation load sensitivity and &lt;5% coefficient of variation inter-\/intra-assay reproducibility. 120 clinical samples were tested by the multiplex snapback primer assay, and verified with amplification refractory system (ARMS), quantitative PCR (qPCR) and Sanger sequencing method. The multiplex system, with a favored versatility, provided the molecular diagnosis of Ph-negative MPNs with a suitable implement and simplified the genetic test process.<\/jats:p>","DOI":"10.1155\/2014\/458457","type":"journal-article","created":{"date-parts":[[2014,3,9]],"date-time":"2014-03-09T05:21:35Z","timestamp":1394342495000},"page":"1-11","source":"Crossref","is-referenced-by-count":3,"title":["A Multiplex Snapback Primer System for the Enrichment and Detection of<i>JAK2<\/i>V617F and<i>MPL<\/i>W515L\/K Mutations in Philadelphia-Negative Myeloproliferative Neoplasms"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0466-5996","authenticated-orcid":true,"given":"Zhiyuan","family":"Wu","sequence":"first","affiliation":[{"name":"Central Laboratory, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China"},{"name":"Department of Laboratory Medicine, Huashan Hospital North, Shanghai Medical College, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinju","family":"Zhang","sequence":"additional","affiliation":[{"name":"Central Laboratory, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Central Laboratory, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihua","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shibao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Huashan Hospital North, Shanghai Medical College, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8796-2653","authenticated-orcid":true,"given":"Ming","family":"Guan","sequence":"additional","affiliation":[{"name":"Central Laboratory, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China"},{"name":"Department of Laboratory Medicine, Huashan Hospital North, Shanghai Medical College, Fudan University, Shanghai, China"},{"name":"Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20009"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.03.023"},{"key":"3","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1016\/S0140-6736(05)74230-6","volume":"365","year":"2005","journal-title":"The Lancet"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-1320"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051113"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030270"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-04-018879"},{"key":"8","year":"2008"},{"issue":"17","key":"9","doi-asserted-by":"crossref","first-page":"4567","DOI":"10.1093\/nar\/20.17.4567","volume":"20","year":"1992","journal-title":"Nucleic Acids Research"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07560.x"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0334858100"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-12-257501"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.142034"},{"issue":"1","key":"14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0378-1119(89)90358-2","volume":"77","year":"1989","journal-title":"Gene"},{"key":"15","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1073\/pnas.0305476101","volume":"101","year":"2004","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2009.10.012"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2007.060083"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2008.080015"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404955"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-209262"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s11899-009-0005-6"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1110556"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1110557"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.031153"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404325"},{"key":"26","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1038\/sj.leu.2404654","volume":"21","year":"2007","journal-title":"Leukemia"},{"issue":"10","key":"27","first-page":"967","volume":"366","year":"2012","journal-title":"The New England Journal of Medicine"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2010.08083.x"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.137"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.107615"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2011.166306"},{"key":"32","year":"2012"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPFHUQZ9AGUEKA"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2010.08.020"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2011.04.012"},{"key":"36","first-page":"1582","volume":"97","year":"2012","journal-title":"Haematologica"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1373\/49.6.853"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2010.034793"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026534"},{"issue":"1","key":"40","first-page":"76","volume":"10","year":"1991","journal-title":"BioTechniques"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/458457.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/458457.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/458457.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T05:17:18Z","timestamp":1498108638000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/458457\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":40,"alternative-id":["458457","458457"],"URL":"https:\/\/doi.org\/10.1155\/2014\/458457","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}