{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T21:38:42Z","timestamp":1769549922250,"version":"3.49.0"},"reference-count":72,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"crossref","award":["2011\/11550-3"],"award-info":[{"award-number":["2011\/11550-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"crossref","award":["2012\/15036-8"],"award-info":[{"award-number":["2012\/15036-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"crossref","award":["2011\/11550-3"],"award-info":[{"award-number":["2011\/11550-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"crossref","award":["2012\/15036-8"],"award-info":[{"award-number":["2012\/15036-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Objective.<\/jats:italic>Annexin-A1 (<jats:italic>ANXA1<\/jats:italic>\/AnxA1) and galectin-1 (<jats:italic>LGALS1<\/jats:italic>\/Gal-1) are mediators that play an important role in the inflammatory response and are also associated with carcinogenesis. We investigated mRNA and protein expression in precancerous gastric lesions that participate in the progression cascade to gastric cancer, such as intestinal metaplasia (IM) and gastric ulcer (GU).<jats:italic>Methods.<\/jats:italic>Quantitative real-time PCR (qPCR) and immunohistochemical techniques were used to analyze the relative quantification levels (RQ) of<jats:italic>ANXA1<\/jats:italic>and<jats:italic>LGALS1<\/jats:italic>mRNA and protein expression, respectively.<jats:italic>Results.<\/jats:italic>Increased relative expression levels of<jats:italic>ANXA1<\/jats:italic>were found in 100% of cases, both in IM (mean<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mtext>RQ<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn>6.22<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.06<\/mml:mn><\/mml:math>) and in GU (mean<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mtext>RQ<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn>6.69<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.10<\/mml:mn><\/mml:math>). However, the<jats:italic>LGALS1<\/jats:italic>presented basal expression in both groups (IM: mean<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mtext>RQ<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn>0.35<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.07<\/mml:mn><\/mml:math>; GU: mean<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mtext>RQ<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn>0.69<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.09<\/mml:mn><\/mml:math>). Immunohistochemistry revealed significant positive staining for both the AnxA1 and Gal-1 proteins in the epithelial nucleus and cytoplasm as well as in the stroma of the IM and GU groups (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>) but absence or low immunorectivity in normal mucosa.<jats:italic>Conclusion<\/jats:italic>. Our results bring an important contribution by evidencing that both the AnxA1 and Gal-1 anti-inflammatory proteins are deregulated in precancerous gastric lesions, suggesting their involvement in the early stages of gastric carcinogenesis, possibly due to an inflammatory process in the gastric mucosa.<\/jats:p>","DOI":"10.1155\/2014\/478138","type":"journal-article","created":{"date-parts":[[2014,2,26]],"date-time":"2014-02-26T01:09:24Z","timestamp":1393376964000},"page":"1-11","source":"Crossref","is-referenced-by-count":19,"title":["Deregulation of Annexin-A1 and Galectin-1 Expression in Precancerous Gastric Lesions: Intestinal Metaplasia and Gastric Ulcer"],"prefix":"10.1155","volume":"2014","author":[{"given":"Ana Fl\u00e1via Teixeira","family":"Rossi","sequence":"first","affiliation":[{"name":"Department of Biology, S\u00e3o Paulo State University (UNESP), C\u00e2mpus S\u00e3o Jos\u00e9 do Rio Preto, Rua Crist\u00f3v\u00e3o Colombo 2265, 15054-000 S\u00e3o Jos\u00e9 do Rio Preto, 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