{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:49:27Z","timestamp":1788871767414,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Office of vice-chancellor for research, Shiraz University of Medical Sciences","award":["3602"],"award-info":[{"award-number":["3602"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Interdisciplinary Perspectives on Infectious Diseases"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>\n                    Serological assays have been extensively evaluated for diagnosis of visceral leishmaniasis (VL) and considered as a routine method for diagnosis of VL while these methods are not properly evaluated for diagnosis of cutaneous leishmaniasis (CL). This study aimed to assess the performance of indirect immunofluorescent-antibody test (IFA) and enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of cutaneous leishmaniasis in Iran. Sixty-one sera samples from parasitologically confirmed CL patients and 50 sera from healthy controls along with 50 sera from non-CL patients were collected. Antigen was prepared from promastigotes and amastigotes of\n                    <jats:italic>Leishmania major<\/jats:italic>\n                    . IFA was used to detect anti-\n                    <jats:italic>Leishmania<\/jats:italic>\n                    IgG while ELISA was used to detect anti-\n                    <jats:italic>Leishmania<\/jats:italic>\n                    IgM, total IgG, or IgG subclasses (IgG1 and 4). ELISA, for detection of total IgG and IgM, showed sensitivity of 83.6% and 84.7% and specificity of 62.7% and 54.6%, respectively. Sensitivity and specificity of ELISA for detecting IgG1 and IgG4 were 64%, 75% and 85%, 49%, respectively. Sensitivity and specificity of IFA were 91.6% and 81%.\n                    <jats:italic>Conclusion<\/jats:italic>\n                    . Findings of this study demonstrated that serological test, especially IFA, can be used for proper diagnosis of CL.\n                  <\/jats:p>","DOI":"10.1155\/2014\/505134","type":"journal-article","created":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T17:06:50Z","timestamp":1407776810000},"page":"1-4","source":"Crossref","is-referenced-by-count":26,"title":["Performance of an ELISA and Indirect Immunofluorescence Assay in Serological Diagnosis of Zoonotic Cutaneous Leishmaniasis in Iran"],"prefix":"10.1155","volume":"2014","author":[{"given":"Bahador","family":"Sarkari","sequence":"first","affiliation":[{"name":"Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzieh","family":"Ashrafmansouri","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, 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