{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T15:52:21Z","timestamp":1721058741061},"reference-count":36,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Electrophoretic analysis of various developmental stage specific somatic antigens of<jats:italic>Paramphistomum epiclitum<\/jats:italic>(Digenea: Paramphistomidae), namely, metacercariae (McAg), immature intestinal flukes (ImIAg), immature ruminal flukes (ImRAg), and adult flukes (AAg), was done by native polyacrylamide gel electrophoresis. Result revealed presence of 3 (range 15.2\u201340.3\u2009kDa), 13 (9.3\u2013121.2\u2009kDa), 14 (9.3\u2013169.3\u2009kDa), and 15 (8.0\u2013169.3\u2009kDa) polypeptides in McAg, ImIAg, ImRAg, and AAg, respectively. With an aim to identify a suitable immunodiagnostic antigen for early diagnosis of amphistomosis, the IgG antibody response to various developmental stage antigens in goats experimentally infected with metacercariae of<jats:italic>P. epiclitum<\/jats:italic>was evaluated by ELISA. The highest OD values were recorded with ImIAg which ranged between 0.23 and 0.55 with a significant increase from the 2nd week till 8th week of infection with a peak at 6th week. The analysis of statistical significance using a one-way analysis of variance with multiple pair wise comparisons revealed that IgG response was significantly higher with all antigens (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mtext>0.01<\/mml:mtext><\/mml:math>) except McAg (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&gt;<\/mml:mo><mml:mtext>0.05<\/mml:mtext><\/mml:math>) with a maximum mean difference of 0.1838 in comparison to control with ImIAg, thus, indicating that ImIAg which could be further exploited for its potential is a candidate for immunodiagnostic antigen for early diagnosis of amphistomosis.<\/jats:p>","DOI":"10.1155\/2014\/505484","type":"journal-article","created":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T21:06:44Z","timestamp":1402002404000},"page":"1-6","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of Antibody Response to Various Developmental Stage Specific Somatic Antigens of<i>Paramphistomum epiclitum<\/i>in Goats"],"prefix":"10.1155","volume":"2014","author":[{"family":"Jyoti","sequence":"first","affiliation":[{"name":"Department of Veterinary Parasitology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana 141004, India"},{"name":"Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Prasad","sequence":"additional","affiliation":[{"name":"Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nirbhay Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana 141004, India"},{"name":"Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"key":"1","volume":"43, article 272","year":"1972","journal-title":"Indian Journal of Animal Science"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-008-9046-x"},{"issue":"2","key":"3","first-page":"168","volume":"25","year":"2011","journal-title":"Journal of Veterinary Parasitology"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-012-0119-y"},{"issue":"1","key":"5","first-page":"33","volume":"31","year":"1970","journal-title":"Rivista di Parassitologia"},{"issue":"23","key":"6","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1136\/vr.146.23.674","volume":"146","year":"2000","journal-title":"Veterinary Record"},{"issue":"6","key":"7","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1111\/j.1751-0813.1996.tb07569.x","volume":"74","year":"1996","journal-title":"Australian Veterinary Journal"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.04.008"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.2478\/s11687-008-0012-5"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00326-5"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.04.007"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.08.019"},{"issue":"1","key":"13","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1080\/09712119.1999.9706262","volume":"16","year":"1999","journal-title":"Journal of Applied Animal Research"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00192-8"},{"issue":"2","key":"15","first-page":"117","volume":"21","year":"2007","journal-title":"Journal of Veterinary Parasitology"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-010-0011-6"},{"issue":"2","key":"17","first-page":"32","volume":"4","year":"2009","journal-title":"Iranian Journal of Parasitology"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.03.016"},{"key":"19","first-page":"568","volume":"64","year":"1994","journal-title":"Indian Journal of Animal Science"},{"key":"20","first-page":"149","volume":"20","year":"2003","journal-title":"Rivista di Parassitologia"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/BF00009622"},{"issue":"5","key":"22","doi-asserted-by":"crossref","first-page":"791","DOI":"10.2307\/3280984","volume":"68","year":"1982","journal-title":"Journal of Parasitology"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(94)00721-N"},{"issue":"1","key":"24","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","year":"1951","journal-title":"The Journal of Biological Chemistry"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"issue":"2","key":"26","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S0034-5288(18)30788-4","volume":"43","year":"1987","journal-title":"Research in Veterinary Science"},{"issue":"4","key":"27","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/0020-7519(88)90016-1","volume":"18","year":"1988","journal-title":"International Journal of Parasitology"},{"key":"28","first-page":"165","volume":"11","year":"1997","journal-title":"Journal of Veterinary Parasitology"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1007\/BF00012268"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1023\/B:VERC.0000047497.57392.8c"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-0941-4"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-176"},{"issue":"1\u20134","key":"33","first-page":"313","volume":"17","year":"1987","journal-title":"Veterinary Immunology and Immunopathology"},{"key":"34","first-page":"72","volume":"28","year":"1981","journal-title":"Ankara Universities Veteriner Fakultesi Dergisi"},{"key":"35","volume":"54, article 820","year":"1983","journal-title":"Veterinary Bulletin Abstracts"},{"key":"36","first-page":"338","volume":"96","year":"1983","journal-title":"Berlin and Munchener Tierarztliche Wochenschrift"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/505484.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/505484.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/505484.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T04:56:44Z","timestamp":1649221004000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/505484\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":36,"alternative-id":["505484","505484"],"URL":"https:\/\/doi.org\/10.1155\/2014\/505484","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}