{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T07:13:11Z","timestamp":1768720391052,"version":"3.49.0"},"reference-count":29,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>This study analyzed the association of the A\/G SNP at position +49 of exon-1 in the CTLA-4 gene to the susceptibility and clinical manifestations of Behcet\u2019s disease (BD). It was performed on 60 Egyptian BD patients and 95 age- and sex-matched healthy controls. The genotypes for the +49 A\/G polymorphism of the CTLA-4 gene were determined by PCR-RFLP, while the serum level of CTLA-4 protein was measured by ELISA. CTLA-4 +49 A allele (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mtext>OR<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">3.084<\/mml:mn><\/mml:math>, and CI (95%) = 1.90\u20134.99) and A\/A genotype (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mtext>OR<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">6.643<\/mml:mn><\/mml:math>, and CI (95%) = 2.58\u201317.10) frequency distribution was significantly more increased in patients than in the controls, with no significant differences between males and females with regard to the genotype or allele frequency distribution. A\/A genotype was associated with a more reduced expression of sCTLA-4 protein in patients than in the controls (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mn fontstyle=\"italic\">1.76<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.19<\/mml:mn><\/mml:math>versus<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mn fontstyle=\"italic\">1.91<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.30<\/mml:mn><\/mml:math>, resp;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.0007<\/mml:mn><\/mml:math>). In addition, it is associated with the occurrence of ocular and vasculitic manifestations of BD in the patient group. The CTLA-4 gene could be considered as a susceptibility and a disease-modifying gene to BD in Egyptian population that needs further confirmatory studies on larger cohorts.<\/jats:p>","DOI":"10.1155\/2014\/513915","type":"journal-article","created":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T18:52:00Z","timestamp":1401735120000},"page":"1-6","source":"Crossref","is-referenced-by-count":8,"title":["The Role of CTLA-4 Exon-1 49 A\/G Polymorphism and Soluble CTLA-4 Protein Level in Egyptian Patients with Beh\u00e7et's Disease"],"prefix":"10.1155","volume":"2014","author":[{"given":"Sahar M.","family":"Abdel Galil","sequence":"first","affiliation":[{"name":"Rheumatology & Rehabilitation Department, Faculty of Medicine, Zagazig University, Zagazig 44511, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoda A.","family":"Hagrass","sequence":"additional","affiliation":[{"name":"Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig 44511, 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