{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T15:40:08Z","timestamp":1788968408692,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Infectious Diseases of China","award":["2012ZX10004215"],"award-info":[{"award-number":["2012ZX10004215"]}]},{"name":"Infectious Diseases of China","award":["2013ZX10004-803"],"award-info":[{"award-number":["2013ZX10004-803"]}]},{"DOI":"10.13039\/501100003711","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["2012ZX10004215"],"award-info":[{"award-number":["2012ZX10004215"]}],"id":[{"id":"10.13039\/501100003711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003711","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["2013ZX10004-803"],"award-info":[{"award-number":["2013ZX10004-803"]}],"id":[{"id":"10.13039\/501100003711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>It is critical to develop a cost-effective detection kit for rapid diagnosis and on-site detection of severe fever with thrombocytopenia syndrome virus (SFTSV) infection. Here, an immunochromatographic assay (ICA) to detect SFTSV infection is described. The ICA uses gold nanoparticles coated with recombinant SFTSV for the simultaneous detection of IgG and IgM antibodies to SFTSV. The ICA was developed and evaluated by using positive sera samples of SFTSV infection<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mo stretchy=\"false\">(<\/mml:mo><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>245<\/mml:mn><mml:mo stretchy=\"false\">)<\/mml:mo><\/mml:math>collected from the CDC of China. The reference laboratory diagnosis of SFTSV infection was based on the \u201cgold standard\u201d. The results demonstrated that the positive coincidence rate and negative coincidence rate were determined to be 98.4% and 100% for IgM and 96.7% and 98.6% for IgG, respectively. The kit showed good selectivity for detection of SFTSV-specific IgG and IgM with no interference from positive sera samples of Japanese encephalitis virus infection, Dengue virus infection, Hantavirus infection, HIV infection, HBV surface antigen, HCV antibody,<jats:italic>Mycobacterium tuberculosis<\/jats:italic>antibody, or RF. Based on these results, the ICS test developed may be a suitable tool for rapid on-site testing for SFTSV infections.<\/jats:p>","DOI":"10.1155\/2014\/530621","type":"journal-article","created":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T17:03:50Z","timestamp":1397495030000},"page":"1-6","source":"Crossref","is-referenced-by-count":9,"title":["Development of a Colloidal Gold Kit for the Diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus Infection"],"prefix":"10.1155","volume":"2014","author":[{"given":"Xianguo","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, 1838 N. Guangzhou Avenue, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanfu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Medical Virology, MOH, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Hao","sequence":"additional","affiliation":[{"name":"Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, 1838 N. Guangzhou Avenue, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunian","family":"Gao","sequence":"additional","affiliation":[{"name":"DaAn Gene Co. 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Ltd. of Sun Yat-sen University, 19 Xiangshan Road, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9058-4833","authenticated-orcid":true,"given":"Shuhong","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, 1838 N. Guangzhou Avenue, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwen","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, 1838 N. 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