{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:53:25Z","timestamp":1773924805497,"version":"3.50.1"},"reference-count":38,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Foundation to support the research of Amazonas State (FAPEAM)"},{"name":"Foundation to support the research of Minas Gerais State (FAPEMIG)"},{"name":"National Council for Scientific and Technological Development (CNPq)"},{"name":"Coordination of Improvement of Higher Education Personnel (CAPES)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Shigellosis is a global human health problem and the incidence is highest among children. In the present work, main<jats:italic>Shigella<\/jats:italic>virulence genes was examined by PCR and compared to symptoms of pediatric shigellosis. Thirty<jats:italic>Shigella<\/jats:italic>isolates were identified from an etiologic study at which 1,339 children ranging 0\u201310 years old were enrolled.<jats:italic>S. flexneri<\/jats:italic>was the most frequent species reaching 60.0% of isolates, 22.2% were<jats:italic>S. sonnei<\/jats:italic>, and 6.6% were both<jats:italic>S. dysenteriae<\/jats:italic>and<jats:italic>S. boydii<\/jats:italic>. All<jats:italic>Shigella<\/jats:italic>infected children had diarrhea, but not all were accompanied by others symptoms of bacillary dysentery. Among major virulence genes, the PCR typing revealed<jats:italic>ipaBCD<\/jats:italic>was present in all isolates, followed by<jats:italic>IpaH7.8<\/jats:italic>,<jats:italic>set-1A<\/jats:italic>,<jats:italic>set-1B, sen\/ospD3, virF,<\/jats:italic>and<jats:italic>invE<\/jats:italic>. The pathogenic potential of the ShET-1B subunit was observed in relation to dehydration (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>) and ShET-2 related to the intestinal injury (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.033<\/mml:mn><\/mml:math>) evidenced by the presence of bloody diarrhea. Our results show associations among symptoms of shigellosis and virulence genes of clinical isolates of<jats:italic>Shigella<\/jats:italic>spp.<\/jats:p>","DOI":"10.1155\/2014\/539697","type":"journal-article","created":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T21:07:52Z","timestamp":1398805672000},"page":"1-9","source":"Crossref","is-referenced-by-count":29,"title":["Virulence Factors Associated with Pediatric Shigellosis in Brazilian Amazon"],"prefix":"10.1155","volume":"2014","author":[{"given":"Carolinie Batista Nobre da","family":"Cruz","sequence":"first","affiliation":[{"name":"Instituto Le\u00f4nidas e Maria Deane\u2014Fiocruz Amaz\u00f4nia, Rua Terezina 476, Adrian\u00f3polis, 69.057-070 Manaus, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Carolina Scheffer de","family":"Souza","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada (PPGBA-UFAM), Avenida General 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Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio Alessandro","family":"Pieri","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal de Juiz de Fora, C\u00e2mpus Governador Valadares, Rua Israel Pinheiro 2000, Bairro Universit\u00e1rio, 35010177 Governador Valadares, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Afonso","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Instituto Le\u00f4nidas e Maria Deane\u2014Fiocruz Amaz\u00f4nia, Rua Terezina 476, Adrian\u00f3polis, 69.057-070 Manaus, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia Puccinelli","family":"Orlandi","sequence":"additional","affiliation":[{"name":"Instituto Le\u00f4nidas e Maria Deane\u2014Fiocruz Amaz\u00f4nia, Rua Terezina 476, Adrian\u00f3polis, 69.057-070 Manaus, AM, 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