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The present study investigates the effects of allyl isothiocyanate (AITC) on<jats:italic>MRP1<\/jats:italic>mRNA and MRP1 protein expression and transporter activity in the immortalised human bronchial epithelial cell line 16HBE14o-.<jats:italic>MRP1<\/jats:italic>mRNA and MRP1 protein expression in 16HBE14o- cells that were treated with allyl isothiocyanate were analysed by real-time PCR assay and Western blotting. The transport of carboxyfluorescein, a known MRP1 substrate, was measured by functional flow cytometry to evaluate MRP1 activity. Treatment with AITC at concentrations of 5\u201340\u2009<jats:italic>\u03bc<\/jats:italic>M increased MRP1 protein levels in a concentration-dependent manner. AITC treatments at concentrations of 1\u201340\u2009<jats:italic>\u03bc<\/jats:italic>M caused concentration-dependent increases in<jats:italic>MRP1<\/jats:italic>mRNA levels that were up to seven times greater than the levels found in control cells. Finally, AITC treatment at concentrations of 5\u201340\u2009<jats:italic>\u03bc<\/jats:italic>M significantly increased MRP1-dependent efflux in 16HBE14o- cells. These results suggest that AITC can increase the expression and activity of MRP1 in 16HBE14o- cells in a concentration-dependent manner. The upregulation of MRP1 activity and expression by AITC could produce therapeutic effects in the treatment of lung disease.<\/jats:p>","DOI":"10.1155\/2014\/547379","type":"journal-article","created":{"date-parts":[[2014,1,14]],"date-time":"2014-01-14T21:02:10Z","timestamp":1389733330000},"page":"1-7","source":"Crossref","is-referenced-by-count":12,"title":["Allyl Isothiocyanate Increases MRP1 Function and Expression in a Human Bronchial Epithelial Cell Line"],"prefix":"10.1155","volume":"2014","author":[{"given":"Dian-lei","family":"Wang","sequence":"first","affiliation":[{"name":"Anhui University of Chinese Medicine, Hefei, Anhui 230038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen-yin","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui University of Chinese Medicine, Hefei, Anhui 230038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Cao","sequence":"additional","affiliation":[{"name":"The 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