{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:52:14Z","timestamp":1772826734246,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Gobierno de Arag\u00f3n and Fondo Social Europeo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Nematicidal activity of<jats:italic>Melaleuca alternifolia<\/jats:italic>essential oil, commonly known as tea tree oil (TTO), was assayed<jats:italic>in vitro<\/jats:italic>against L3 larvae of<jats:italic>Anisakis simplex<\/jats:italic>. The results showed a mortality of 100% for concentrations between 7 and 10\u2009<jats:italic>\u03bc<\/jats:italic>L\/mL after 48\u2009h of incubation, obtaining an LD50 value of 4.53\u2009<jats:italic>\u03bc<\/jats:italic>L\/mL after 24 hours and 4.27\u2009<jats:italic>\u03bc<\/jats:italic>L\/mL after 48 hours. Concentration-dependent inhibition of acetylcholinesterase was observed for tea tree essential oil showing inhibition values of 100% at 100\u2009<jats:italic>\u03bc<\/jats:italic>L\/mL. This fact suggests that TTO may act as an AChE inhibitor. Terpinen-4-ol was discarded as main larvicide compound as it did not show larvicidal or anticholinesterase activity. The data obtained suggest that the essential oil of<jats:italic>Melaleuca alternifolia<\/jats:italic>may have a great therapeutic potential for the treatment of human anisakiasis.<\/jats:p>","DOI":"10.1155\/2014\/549510","type":"journal-article","created":{"date-parts":[[2014,5,25]],"date-time":"2014-05-25T21:07:17Z","timestamp":1401052037000},"page":"1-6","source":"Crossref","is-referenced-by-count":25,"title":["Activity of Tea Tree (<i>Melaleuca alternifolia<\/i>) Essential Oil against L3 Larvae of<i>Anisakis simplex<\/i>"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3982-2282","authenticated-orcid":true,"given":"Carlota","family":"G\u00f3mez-Rinc\u00f3n","sequence":"first","affiliation":[{"name":"Facultad de Ciencias de la Salud, Universidad San Jorge, Campus Universitario Villanueva de G\u00e1llego, Autovia A-23 Zaragoza, Huesca, Km 299, 50.830 Villanueva de G\u00e1llego, Zaragoza, 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