{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,6]],"date-time":"2024-04-06T22:36:07Z","timestamp":1712442967655},"reference-count":36,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Mycoplasmas are fastidious slow growing organisms lacking a cell wall and mostly isolated from the mucosal surfaces of the respiratory and genitourinary tracts. There is a dearth of information regarding clinical<jats:italic>Mycoplasma<\/jats:italic>spp. isolates among Egyptian patients. A total of 170 samples were collected from patients and apparently healthy personnel in local public hospitals in Cairo, Egypt. Isolation of<jats:italic>Mycoplasma<\/jats:italic>spp. was carried out using appropriate culture media and further identification was carried out by biochemical tests followed by serotyping using specific antisera. Confirmation was done by PCR for detection of different<jats:italic>Mycoplasma<\/jats:italic>spp. using genus-specific primers targeting 16S ribosomal RNA gene. Characterization of the antibiotic resistance and sensitivity pattern against different antimicrobials was carried out using disc diffusion test. The results indicated the presence of six<jats:italic>Mycoplasma<\/jats:italic>spp. in 22.94% of the samples. Mycoplasmas were detected more frequently in throat swabs than sputum.<jats:italic>Mycoplasma pneumoniae<\/jats:italic>was highly sensitive to macrolides and quinolones but less sensitive to aminoglycosides and tetracyclines. Molecular techniques were found to be of more rapid, highly sensitive, able to detect nonviable organisms, and cost effective. These results shed light on difficulties of<jats:italic>Mycoplasma<\/jats:italic>detection and the superiority of molecular techniques over culture.<\/jats:p>","DOI":"10.1155\/2014\/549858","type":"journal-article","created":{"date-parts":[[2014,11,23]],"date-time":"2014-11-23T21:00:42Z","timestamp":1416776442000},"page":"1-6","source":"Crossref","is-referenced-by-count":6,"title":["Detection, Characterization, and Molecular Typing of Human<i>Mycoplasma<\/i>spp. from Major Hospitals in Cairo, Egypt"],"prefix":"10.1155","volume":"2014","author":[{"given":"Mirihan A.","family":"Metwally","sequence":"first","affiliation":[{"name":"Department of Microbiology, National Organization for Drug Control and Research (NODCAR), Giza 12561, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aymen S.","family":"Yassin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr Eleini Street, Cairo 11562, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamer M.","family":"Essam","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr Eleini Street, Cairo 11562, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayam M.","family":"Hamouda","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Organization for Drug Control and Research (NODCAR), Giza 12561, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdy A.","family":"Amin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr Eleini Street, Cairo 11562, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/17.Supplement_1.S10"},{"issue":"4","key":"2","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1128\/MMBR.62.4.1094-1156.1998","volume":"62","year":"1998","journal-title":"Microbiology and Molecular Biology Reviews"},{"key":"3","year":"2012"},{"key":"4","volume-title":"Current status of the mollicute (Mycoplasma) lung disease: pathogenesis, diagnostics, treatment and prevention","year":"2012"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.3201\/eid0301.970103"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2012.06.001"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1300\/J092v11n02_03"},{"key":"8","first-page":"383","volume":"2","year":"2005","journal-title":"Medical Veritas"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197301112880206"},{"key":"10","series-title":"Mycoplasma Protocols","volume":"104","year":"1998"},{"key":"11","first-page":"45","volume-title":"Mycoplasmas of humans","year":"2002"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.11.5.862-867.2004"},{"issue":"2","key":"13","first-page":"88","volume":"4","year":"2010","journal-title":"Egyptian Journal of Bronchology"},{"key":"14","volume":"2","year":"1983"},{"issue":"1","key":"15","first-page":"104","volume":"225","year":"1973","journal-title":"Zentralblatt f\u00fcr Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene"},{"issue":"3","key":"16","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1186\/BF03547432","volume":"14","year":"1973","journal-title":"Acta Veterinaria Scandinavica"},{"issue":"3","key":"17","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1128\/AEM.23.3.553-558.1972","volume":"23","year":"1972","journal-title":"Applied microbiology"},{"key":"18","year":"1994"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(95)00254-5"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.2307\/1592409"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00058-I"},{"key":"23","series-title":"Mycoplasma Protocols","first-page":"69","volume-title":"Biochemical characteristics in mycoplasma identification","volume":"104","year":"1998"},{"issue":"5","key":"24","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1128\/JCM.32.5.1387-1389.1994","volume":"32","year":"1994","journal-title":"Journal of Clinical Microbiology"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1080\/00034989859294"},{"issue":"3","key":"27","first-page":"279","volume":"9","year":"2002","journal-title":"Eastern Mediterranean Health Journal"},{"issue":"6","key":"28","volume":"17","year":"2012","journal-title":"Eurosurveillance"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-197904000-00004"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-1-291"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1177\/00220345710500065501"},{"key":"32","volume-title":"Mycoplasmas","year":"1994"},{"key":"33","series-title":"edited by F. S. Nolte","year":"2000"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.3.6.635"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.patbio.2004.01.002"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2005.017368"},{"issue":"12","key":"37","doi-asserted-by":"crossref","first-page":"3280","DOI":"10.1128\/JCM.30.12.3280-3283.1992","volume":"30","year":"1992","journal-title":"Journal of Clinical Microbiology"},{"issue":"11","key":"38","first-page":"1401","volume":"30","year":"2009","journal-title":"Saudi Medical Journal"}],"container-title":["The Scientific World Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2014\/549858.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2014\/549858.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2014\/549858.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T04:23:05Z","timestamp":1650687785000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/tswj\/2014\/549858\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":36,"alternative-id":["549858","549858"],"URL":"https:\/\/doi.org\/10.1155\/2014\/549858","relation":{},"ISSN":["2356-6140","1537-744X"],"issn-type":[{"value":"2356-6140","type":"print"},{"value":"1537-744X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}