{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T16:39:34Z","timestamp":1768840774796,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T00:00:00Z","timestamp":1405382400000},"content-version":"vor","delay-in-days":195,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Cooperative Research Program for Agriculture Science & Technology Development","award":["PJ009241"],"award-info":[{"award-number":["PJ009241"]}]},{"DOI":"10.13039\/501100003627","name":"Rural Development Administration, Korea","doi-asserted-by":"crossref","award":["PJ009241"],"award-info":[{"award-number":["PJ009241"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>The inhibitory activities of the <jats:italic>Cordyceps pruinosa<\/jats:italic> butanol fraction (Cp\u2010BF) were investigated by determining inflammatory responses of lipopolysaccharide (LPS)\u2010treated RAW264.7 macrophage cells and by evaluating HCl\/ethanol (EtOH)\u2010triggered gastric ulcers in mice. The molecular mechanisms of the inhibitory effects of Cp\u2010BF were investigated by identifying target enzymes using biochemical and molecular biological approaches. Cp\u2010BF strongly inhibited the production of NO and TNF\u2010<jats:italic>\u03b1<\/jats:italic>, release of reactive oxygen species (ROS), phagocytic uptake of FITC\u2010dextran, and mRNA expression levels of interleukin (IL)\u20106, inducible NO synthase (iNOS), and tumour necrosis factor\u2010alpha (TNF)\u2010<jats:italic>\u03b1<\/jats:italic> in activated RAW264.7 cells. Cp\u2010BF also strongly downregulated the NF\u2010<jats:italic>\u03ba<\/jats:italic>B pathway by suppressing IKK<jats:italic>\u03b2<\/jats:italic> according to luciferase reporter assays and immunoblot analysis. Furthermore, Cp\u2010BF blocked both increased levels of NF\u2010<jats:italic>\u03ba<\/jats:italic>B\u2010mediated luciferase activities and phosphorylation of p65\/p50 observed by IKK<jats:italic>\u03b2<\/jats:italic> overexpression. Finally, orally administered Cp\u2010BF was found to attenuate gastric ulcer and block the phosphorylation of I<jats:italic>\u03ba<\/jats:italic>B<jats:italic>\u03b1<\/jats:italic> induced by HCl\/EtOH. Therefore, these results suggest that the anti\u2010inflammatory activity of Cp\u2010BF may be mediated by suppression of IKK<jats:italic>\u03b1<\/jats:italic> and its downstream NF\u2010<jats:italic>\u03ba<\/jats:italic>B activation. Since our group has established the mass cultivation conditions by developing culture conditions for <jats:italic>Cordyceps pruinosa<\/jats:italic>, the information presented in this study may be useful for developing new anti\u2010inflammatory agents.<\/jats:p>","DOI":"10.1155\/2014\/562467","type":"journal-article","created":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T21:06:35Z","timestamp":1405458395000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["IKK<i>\u03b2<\/i>\u2010Targeted Anti\u2010Inflammatory Activities of a Butanol Fraction of Artificially Cultivated <i>Cordyceps pruinosa<\/i> Fruit Bodies"],"prefix":"10.1155","volume":"2014","author":[{"given":"Han Gyung","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo Seok","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gi-Ho","family":"Sung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Hye","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3164-7379","authenticated-orcid":false,"given":"Gwang-Soo","family":"Baek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunji","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sungjae","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung Chul","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Mo","family":"Sung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deok Hyo","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae Woong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sungyoul","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Hoon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8141-9927","authenticated-orcid":false,"given":"Jae Youl","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,15]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.1.380"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2443.2001.00458.x"},{"key":"e_1_2_9_3_2","first-page":"717","article-title":"Pathogenesis of joint damage in rheumatoid arthritis","volume":"26","author":"Bresnihan B.","year":"1999","journal-title":"Journal of Rheumatology"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400104"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7317"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200004)43:4<775::AID-ANR8>3.0.CO;2-7"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250818"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.57.12.0001"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1211\/jpp\/61.03.0002"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/acm.1998.4.429"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/acm.1998.4.3-289"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lab.2005.11.011"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-011-0036-2"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.2012.01601.x"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-008X(03)00152-2"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.01.049"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.12.034"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.5142\/jgr.2013.37.300"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2012.45.6.018"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.4110\/in.2013.13.4.148"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/529359"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2013.026"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.5142\/jgr.2013.37.293"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2013.044"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.5142\/jgr.2011.35.2.200"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2013.10.006"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-013-0067-9"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.12.013"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-013-0050-5"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.42.123"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.01.034"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/979105"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/512926"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.01.022"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.02.018"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.3109\/08923973.2010.482137"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/102918"},{"key":"e_1_2_9_38_2","first-page":"169","article-title":"Study on effect of Cordyceps sinensis and artemisinin in preventing recurrence of lupus nephritis","volume":"22","author":"Lu L.","year":"2002","journal-title":"Chinese Journal of Modern Developments in Traditional Medicine"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.06.047"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.4489\/MYCO.2010.38.2.118"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.12.012"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090823"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2014\/562467.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2014\/562467.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2014\/562467","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T16:57:24Z","timestamp":1723049844000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2014\/562467"}},"subtitle":[],"editor":[{"given":"Youn Chul","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,1]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1155\/2014\/562467"],"URL":"https:\/\/doi.org\/10.1155\/2014\/562467","archive":["Portico"],"relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1]]},"assertion":[{"value":"2014-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-06-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"562467"}}