{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:31:03Z","timestamp":1762079463949,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de Minas Gerais"},{"DOI":"10.13039\/501100004878","name":"Funda\u00e7\u00e3o de Ensino e Pesquisa de Uberaba","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004878","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>This study aimed to measure the synthesis of Th1 and Th2 cytokines by mononuclear cells after culture with live<jats:italic>T. gondii<\/jats:italic>and identified Th17 (CD4<jats:sup>+<\/jats:sup>) and Tc17 (CD8<jats:sup>+<\/jats:sup>) cells in toxoplasma-seronegative and toxoplasma-seropositive parturient and nonpregnant women. Cytometric bead arrays were used to measure cytokine levels (IL-2, TNF-<jats:italic>\u03b1<\/jats:italic>, IFN-<jats:italic>\u03b3<\/jats:italic>, IL-4, IL-5, and IL-10); immunophenotyping was used to characterize Th17 and Tc17 cells, and the cells were stained with antibodies against CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T cells expressing IL-17. The addition of tachyzoites to cell cultures induced the synthesis of IL-5, IL-10, and TNF-<jats:italic>\u03b1<\/jats:italic>by cells from seronegative parturient women and of IL-5 and IL-10 by cells from seropositive, nonpregnant women. We observed a lower level of IL-17-expressing CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T lymphocytes in cultures of cells from seronegative and seropositive parturient and nonpregnant women that were stimulated with tachyzoites, whereas analysis of the CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T cell populations showed a higher level of CD4<jats:sup>+<\/jats:sup>T cells compared with CD8<jats:sup>+<\/jats:sup>T cells. These results suggest that the cytokine pattern and IL-17-expressing CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T lymphocytes may have important roles in the inflammatory response to<jats:italic>T. gondii<\/jats:italic>, thus contributing to the maintenance of pregnancy and control of parasite invasion and replication.<\/jats:p>","DOI":"10.1155\/2014\/573825","type":"journal-article","created":{"date-parts":[[2014,8,17]],"date-time":"2014-08-17T21:06:17Z","timestamp":1408309577000},"page":"1-7","source":"Crossref","is-referenced-by-count":10,"title":["IL-17-Expressing CD4<sup><b>+<\/b><\/sup>and CD8<sup><b>+<\/b><\/sup>T Lymphocytes in Human Toxoplasmosis"],"prefix":"10.1155","volume":"2014","author":[{"given":"J\u00e9ssica L\u00edver","family":"Alves Silva","sequence":"first","affiliation":[{"name":"Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Tri\u00e2ngulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, 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Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virmondes","family":"Rodrigues J\u00fanior","sequence":"additional","affiliation":[{"name":"Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Tri\u00e2ngulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","first-page":"369","volume":"148","year":"1909","journal-title":"Seances Academy Scientific"},{"year":"2007","key":"2"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.09.005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/15.2.211"},{"issue":"1","key":"5","doi-asserted-by":"crossref","first-page":"286","DOI":"10.4049\/jimmunol.146.1.286","volume":"146","year":"1991","journal-title":"Journal of 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