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Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB\/c) mice challenged with myotropic parasite strains (Brazil and Colombian). Initially, we determined that the coadministration during priming of a DNA plasmid containing the murine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptible male mice against acute lethal infections with these parasites. The prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain. Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block. Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease.\n                  <\/jats:p>","DOI":"10.1155\/2014\/605023","type":"journal-article","created":{"date-parts":[[2014,6,26]],"date-time":"2014-06-26T17:04:27Z","timestamp":1403802267000},"page":"1-13","source":"Crossref","is-referenced-by-count":10,"title":["Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of\n                    <i>Trypanosoma cruzi<\/i>"],"prefix":"10.1155","volume":"2014","author":[{"given":"Adriano Fernando","family":"Ara\u00fajo","sequence":"first","affiliation":[{"name":"Centro de Terapia Celular e Molecular (CTCMol), Escola Paulista de Medicina, UNIFESP, Rua Mirassol, 207 04044-010 S\u00e3o Paulo, SP, Brazil"},{"name":"Departmento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de S\u00e3o Paulo, Rua Mirassol, 207 04044-010 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Biologia Celular, Instituto Oswaldo Cruz (FIOCRUZ), Avenida Brasil, 4365\u2014Manguinhos, 21040-360 Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Fraga","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Gon\u00e7alo Moniz, FIOCRUZ, Rua Waldemar Falc\u00e3o, 121, 40296-710 Salvador, BA, Brazil"},{"name":"Hospital S\u00e3o Rafael, Avenida S\u00e3o Rafael 2152, S\u00e3o Marcos, 41253-190 Salvador, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonatan","family":"Ersching","sequence":"additional","affiliation":[{"name":"Centro de Terapia Celular e Molecular (CTCMol), Escola Paulista de Medicina, UNIFESP, Rua Mirassol, 207 04044-010 S\u00e3o Paulo, SP, Brazil"},{"name":"Departmento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de S\u00e3o Paulo, Rua Mirassol, 207 04044-010 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Ribeiro","family":"Dominguez","sequence":"additional","affiliation":[{"name":"Centro de Terapia Celular e Molecular (CTCMol), Escola Paulista de Medicina, UNIFESP, Rua Mirassol, 207 04044-010 S\u00e3o Paulo, SP, Brazil"},{"name":"Departmento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de S\u00e3o Paulo, Rua Mirassol, 207 04044-010 S\u00e3o Paulo, SP, 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Tostes","family":"Gazzinelli","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Ren\u00e9 Rachou, FIOCRUZ, Avenida Augusto de Lima 1.715, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil"},{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Avenida Presidente Ant\u00f4nio Carlos, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil"},{"name":"Division of Infectious Disease and Immunology, Department of Medicine, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Bruna-Romero","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Campus Universit\u00e1rio da Trindade, 88040-900 Florianopolis, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena 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