{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:34:47Z","timestamp":1791192887313,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2011-0015304"],"award-info":[{"award-number":["2011-0015304"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2011-0030034"],"award-info":[{"award-number":["2011-0030034"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2013-1320070"],"award-info":[{"award-number":["2013-1320070"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2011-0015304"],"award-info":[{"award-number":["2011-0015304"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2011-0030034"],"award-info":[{"award-number":["2011-0030034"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2013-1320070"],"award-info":[{"award-number":["2013-1320070"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["2011-0015304"],"award-info":[{"award-number":["2011-0015304"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["2011-0030034"],"award-info":[{"award-number":["2011-0030034"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["2013-1320070"],"award-info":[{"award-number":["2013-1320070"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>This study investigated the profiling of polycyclic aromatic hydrocarbon- (PAH-) induced genotoxicity in cell lines and zebrafish. Each type of cells displayed different proportionality of apoptosis. Mitochondrial DNA (mtDNA) copy number was dramatically elevated after 5-day treatment of fluoranthene and pyrene. The notable deregulated proteins for PAHs exposure were displayed as follows: lamin-A\/C isoform 3 and annexin A1 for benzopyrene; lamin-A\/C isoform 3 and DNA topoisomerase 2-alpha for pentacene; poly[ADP-ribose] polymerase 1 (PARP-1) for fluoranthene; and talin-1 and DNA topoisomerase 2-alpha for pyrene. Among them, lamin-A\/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study. Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54\u2009hpf with more than 400\u2009nM of benzopyrene. In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells. Lamin-A\/C isoform 3, talin-1, and annexin A1 were identified as universal biomarkers for PAHs exposure. Zebrafish, specifically at embryo stage, showed suitable<jats:italic>in vivo<\/jats:italic>model for monitoring PAHs exposure to hematopoietic tissue and other organs.<\/jats:p>","DOI":"10.1155\/2014\/605135","type":"journal-article","created":{"date-parts":[[2014,7,10]],"date-time":"2014-07-10T21:13:34Z","timestamp":1405026814000},"page":"1-12","source":"Crossref","is-referenced-by-count":22,"title":["Profiling of Biomarkers for the Exposure of Polycyclic Aromatic Hydrocarbons: Lamin-A\/C Isoform 3, Poly[ADP-ribose] Polymerase 1, and Mitochondria Copy Number Are Identified as Universal Biomarkers"],"prefix":"10.1155","volume":"2014","author":[{"given":"Hwan-Young","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea"},{"name":"Brain Korea 21 Plus Project, Chonnam National University Medical School, Gwangju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye-Ran","family":"Kim","sequence":"additional","affiliation":[{"name":"Brain Korea 21 Plus Project, Chonnam National University Medical School, Gwangju, Republic of Korea"},{"name":"Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4946-8512","authenticated-orcid":true,"given":"Min-Gu","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thi Dai","family":"Trang","sequence":"additional","affiliation":[{"name":"Brain Korea 21 Plus Project, Chonnam National University Medical School, Gwangju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee-Jo","family":"Baek","sequence":"additional","affiliation":[{"name":"Environment Health Center for Childhood Leukemia and Cancer, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Dong","family":"Moon","sequence":"additional","affiliation":[{"name":"Environment Health Center for Childhood Leukemia and Cancer, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Hee","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon-Pal","family":"Suh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Wook","family":"Ryang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoon","family":"Kook","sequence":"additional","affiliation":[{"name":"Environment Health Center for Childhood Leukemia and Cancer, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myung-Geun","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea"},{"name":"Brain Korea 21 Plus Project, Chonnam National University Medical School, Gwangju, Republic of Korea"},{"name":"Environment Health Center for Childhood Leukemia and Cancer, Chonnam National University Hwasun Hospital, Hwasun, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.111-a33"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/303468a0"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1136\/oem.2003.008375"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s004200050216"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(90)90054-2"},{"issue":"2","key":"6","first-page":"113","volume":"49","year":"1992","journal-title":"British Journal of Industrial Medicine"},{"issue":"1","key":"7","first-page":"69","volume":"4","year":"1995","journal-title":"Cancer Epidemiology Biomarkers & Prevention"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3109\/00498258209052453"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/0048-9697(79)90032-9"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80230-X"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1007\/s004200100251"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kft102"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/etc.675"},{"key":"14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/dev.123.1.1","volume":"123","year":"1996","journal-title":"Development"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.24.12965"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00482-1"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-060-7_18"},{"issue":"4","key":"18","first-page":"S569","volume":"24","year":"2008","journal-title":"Cadernos de Saude Publica"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/S0048-9697(97)00064-8"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1177\/074823379601200601"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s004200050231"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.95103838"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/0048-9697(90)90326-P"},{"key":"24","first-page":"1","volume":"32","year":"1983","journal-title":"IARC Monographs on the Evaluation of Carcinogenic Risks to Humans"},{"issue":"4","key":"25","first-page":"373","volume":"9","year":"2000","journal-title":"Cancer Epidemiology Biomarkers and Prevention"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1177\/096032719701601006"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.939983"},{"issue":"6","key":"28","first-page":"545","volume":"7","year":"1998","journal-title":"Cancer Epidemiology Biomarkers and Prevention"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/16.5.1079"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(97)00097-3"},{"issue":"1","key":"31","first-page":"3","volume":"7","year":"1998","journal-title":"Cancer Epidemiology Biomarkers and Prevention"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1021\/bi010872t"},{"issue":"13","key":"33","first-page":"3454","volume":"60","year":"2000","journal-title":"Cancer Research"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.43.100901.135828"},{"key":"35","year":"2003"},{"issue":"1","key":"36","first-page":"1","volume":"5","year":"1981","journal-title":"Journal of Environmental Pathology and Toxicology"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-05-0210"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.04.008"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307957200"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/605135.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/605135.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/605135.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,10]],"date-time":"2022-04-10T22:15:23Z","timestamp":1649628923000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/605135\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":39,"alternative-id":["605135","605135"],"URL":"https:\/\/doi.org\/10.1155\/2014\/605135","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}