{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T13:02:39Z","timestamp":1761310959741},"reference-count":25,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Secretaria de Investigacion y Posgrado"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Interdisciplinary Perspectives on Infectious Diseases"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>American cutaneous leishmaniasis includes a spectrum of clinical forms localized cutaneous, diffuse cutaneous, and mucocutaneous leishmaniasis which can be caused by different strains of<jats:italic>Leishmania<\/jats:italic>belonging to the<jats:italic>L. mexicana<\/jats:italic>or<jats:italic>L. braziliensis<\/jats:italic>complexes which may coexist in the same endemic area. We evaluated the PCR-RFLP assay of the ITS1 genes for direct identification of<jats:italic>Leishmania<\/jats:italic>species in 163 clinical samples and 21 Mexican isolates of<jats:italic>Leishmania<\/jats:italic>. In relation to the Mexican isolates of<jats:italic>Leishmania<\/jats:italic>52% displayed a pattern similar to the<jats:italic>L. (L.) mexicana<\/jats:italic>, 5% showed a mixed pattern compatible with<jats:italic>L. (L.) mexicana<\/jats:italic>and<jats:italic>L. (V.) braziliensis<\/jats:italic>, eight with<jats:italic>L. (L.) amazonensis<\/jats:italic>and<jats:italic>L. (L.) mexicana<\/jats:italic>, and one to<jats:italic>L. (V.) braziliensis<\/jats:italic>. Most of the clinical samples, 109\/116 (94%), gave a pattern similar to that of the<jats:italic>L. mexicana<\/jats:italic>, two clinical samples gave similar patterns to that of<jats:italic>Leishmania<\/jats:italic>braziliensis, and 5 samples gave patterns that suggest a coinfection of<jats:italic>L. (L.) mexicana<\/jats:italic>and<jats:italic>L. (V.) braziliensis<\/jats:italic>or<jats:italic>L. (L.) mexicana<\/jats:italic>and<jats:italic>L. (L.) amazonensis<\/jats:italic>. The ITS1 PCR-RFLP assay is a multipurpose tool for diagnosis of<jats:italic>Leishmania<\/jats:italic>from clinical samples and enables determination of the infecting species of New World<jats:italic>Leishmania<\/jats:italic>in the field in relatively short time and low cost.<\/jats:p>","DOI":"10.1155\/2014\/607287","type":"journal-article","created":{"date-parts":[[2014,7,2]],"date-time":"2014-07-02T08:54:58Z","timestamp":1404291298000},"page":"1-6","source":"Crossref","is-referenced-by-count":24,"title":["ITS1 PCR-RFLP Diagnosis and Characterization of<i>Leishmania<\/i>in Clinical Samples and Strains from Cases of Human Cutaneous Leishmaniasis in States of the Mexican Southeast"],"prefix":"10.1155","volume":"2014","author":[{"given":"Amalia","family":"Monroy-Ostria","sequence":"first","affiliation":[{"name":"Escuela Nacional de Ciencias Biol\u00f3gicas, IPN, Carpio y Plan de Ayala, 11340 Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abedelmajeed","family":"Nasereddin","sequence":"additional","affiliation":[{"name":"School of Medicine Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University of Jerusalem, 9112102 Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor M.","family":"Monteon","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biom\u00e9dicas, Universidad Autonoma de Campeche, Patricio Trueba, 24090 Campeche, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Guzm\u00e1n-Bracho","sequence":"additional","affiliation":[{"name":"Instituto de Diagn\u00f3stico y Referencia Epidemiol\u00f3gicos, Francisco P. de Miranda Lomas de Plateros, 01480 Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles L.","family":"Jaffe","sequence":"additional","affiliation":[{"name":"School of Medicine 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