{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:33:28Z","timestamp":1792042408155,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Identification of outer membrane proteins (OMPs) is important to understand the bacteria structure and function, host-pathogen interaction, development of novel vaccine candidates, and diagnostic antigens. But till now the key antigens of<jats:italic>P. multocida<\/jats:italic>B:2 isolate causing haemorrhagic septicaemia (HS) in animals are not clearly defined. In this study, P52 strain of<jats:italic>P. multocida<\/jats:italic>serotype B:2 was grown<jats:italic>in vitro<\/jats:italic>under iron-rich and iron-limited condition. The OMPs were extracted by sarkosyl method followed by SDS-PAGE and the proteins were identified by MALDI-TOF\/MS analysis. In total, 22 proteins were identified, of which 7 were observed exclusively under iron-limited condition. Most of the high molecular weight proteins (TbpA, HgbA, HgbB, HasR, IroA, and HemR) identified in this study were involved in iron acquisition. Some hypothetical proteins (HP-KCU-10206, HP and AAUPMB 08244, HP AAUPMB 21592, HP AAUPMB 19766, AAUPMB 11295) were observed for the first time in this study which could be unique to serotype B:2. Further functional<jats:italic>in vivo<\/jats:italic>study of the proteins identified are required to explore the utility of these proteins in developing diagnostics and vaccine against HS.<\/jats:p>","DOI":"10.1155\/2014\/617034","type":"journal-article","created":{"date-parts":[[2014,12,22]],"date-time":"2014-12-22T21:14:21Z","timestamp":1419282861000},"page":"1-10","source":"Crossref","is-referenced-by-count":13,"title":["Outer Membrane Proteome Analysis of Indian Strain of<i>Pasteurella multocida<\/i>Serotype B:2 by MALDI-TOF\/MS Analysis"],"prefix":"10.1155","volume":"2014","author":[{"given":"A.","family":"Prasannavadhana","sequence":"first","affiliation":[{"name":"Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santosh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Veterinary Bacteriology and 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