{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T09:57:52Z","timestamp":1787911072278,"version":"build-2784847793"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,2,19]],"date-time":"2014-02-19T00:00:00Z","timestamp":1392768000000},"content-version":"vor","delay-in-days":49,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K13202"],"award-info":[{"award-number":["K13202"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K14410"],"award-info":[{"award-number":["K14410"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K14411"],"award-info":[{"award-number":["K14411"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K13202"],"award-info":[{"award-number":["K13202"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K14410"],"award-info":[{"award-number":["K14410"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K14411"],"award-info":[{"award-number":["K14411"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K13202"],"award-info":[{"award-number":["K13202"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K14410"],"award-info":[{"award-number":["K14410"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","award":["K14411"],"award-info":[{"award-number":["K14411"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>\n                    <jats:italic>Ethnopharmacological Relevance<\/jats:italic>\n                    .\n                    <jats:italic>Morus alba<\/jats:italic>\n                    L. leaves (MAE) have been used in fork medicine for the treatment of beriberi, edema, diabetes, hypertension, and atherosclerosis. However, underlying mechanism of MAE on cardiovascular protection remains to be elucidated. Therefore, we investigated whether MAE affect platelet aggregation and thrombosis.\n                    <jats:italic>Materials and Methods<\/jats:italic>\n                    . The anti\u2010platelet activity of MAE was studied using rat platelets. The extent of anti\u2010platelet activity of MAE was assayed in collagen\u2010induced platelet aggregation. ATP and serotonin release was carried out. The activation of integrin\n                    <jats:italic>\u03b1<\/jats:italic>\n                    <jats:sub>IIb<\/jats:sub>\n                    <jats:italic>\u03b2<\/jats:italic>\n                    <jats:sub>3<\/jats:sub>\n                    and phosphorylation of signaling molecules, including MAPK and Akt, were investigated with cytofluorometer and immunoblotting, respectively. The thrombus formation\n                    <jats:italic>in vivo<\/jats:italic>\n                    was also evaluated in arteriovenous shunt model of rats.\n                    <jats:italic>Results<\/jats:italic>\n                    . HPLC chromatographic analysis revealed that MAE contained rutin and isoquercetin. MAE dose\u2010dependently inhibited collagen\u2010induced platelet aggregation. MAE also attenuated serotonin secretion and thromboxane A\n                    <jats:sub>2<\/jats:sub>\n                    formation. In addition, the extract\n                    <jats:italic>in vivo<\/jats:italic>\n                    activity showed that MAE at 100, 200, and 400\u2009mg\/kg significantly and dose\u2010dependently attenuated thrombus formation in rat arterio\u2010venous shunt model by 52.3% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), 28.3% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01), and 19.1% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05), respectively.\n                    <jats:italic>Conclusions<\/jats:italic>\n                    . MAE inhibit platelet activation, TXB2 formation, serotonin secretion, aggregation, and thrombus formation. The plant extract could be considered as a candidate to anti\u2010platelet and antithrombotic agent.\n                  <\/jats:p>","DOI":"10.1155\/2014\/639548","type":"journal-article","created":{"date-parts":[[2014,2,19]],"date-time":"2014-02-19T17:09:02Z","timestamp":1392829742000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Antiplatelet Activity of\n                    <i>Morus alba<\/i>\n                    Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular\u2010Signal\u2010Regulated Kinase and Akt"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1711-0906","authenticated-orcid":false,"given":"Dong-Seon","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5099-0765","authenticated-orcid":false,"given":"Hyun Dong","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man Hee","family":"Rhee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon-Young","family":"Sung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Kyung","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Hyung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho-Kyoung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,19]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61356-7"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.RVI.0000137978.15352.C6"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.50.2.127"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.80.1.198"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/07853890903260898"},{"key":"e_1_2_8_6_2","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1182\/asheducation-2009.1.267","article-title":"Antiplatelet agents","author":"Varon D.","year":"2009","journal-title":"Hematology"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.126.3_suppl.234S"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd985"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00045391-200111000-00009"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(02)03014-0"},{"key":"e_1_2_8_11_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3121\/cmr.1.2.101","article-title":"Antiplatelet therapy from clinical trials to clinical practice","volume":"1","author":"Rezkalla S. H.","year":"2003","journal-title":"Clinical Medicine & Research"},{"key":"e_1_2_8_12_2","volume-title":"Dongeuibogam","author":"Heo J.","year":"2012"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/78.3.544S"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf103661v"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2009.06.002"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.11.143"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.01.020"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.4096"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.4162\/nrp.2009.3.4.272"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.05.055"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(02)00753-7"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X12500279"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.5551\/jat.10058"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.5142\/jgr.2012.36.1.40"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.09532011.09629309"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/485262"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(89)90050-X"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1646657"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2008.02.008"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/895729"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.12136"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.11.005"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.07.049"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.077602"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20061584"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106129200"},{"key":"e_1_2_8_37_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-03-0935"},{"key":"e_1_2_8_38_2","doi-asserted-by":"publisher","DOI":"10.2165\/00129784-200505060-00007"},{"key":"e_1_2_8_39_2","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/S0735-1097(10)80294-3","article-title":"Effects of serotonin and thromboxane A2 on the coronary collateral circulation","volume":"19","author":"Buja L. M.","year":"1992","journal-title":"Journal of the American College of Cardiology"},{"key":"e_1_2_8_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1981.tb07245.x"},{"key":"e_1_2_8_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(90)90652-6"},{"key":"e_1_2_8_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3841.2011.02262.x"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2014\/639548.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2014\/639548.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2014\/639548","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T12:43:18Z","timestamp":1723034598000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2014\/639548"}},"subtitle":[],"editor":[{"given":"Joen-Rong","family":"Sheu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,1]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1155\/2014\/639548"],"URL":"https:\/\/doi.org\/10.1155\/2014\/639548","archive":["Portico"],"relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1]]},"assertion":[{"value":"2013-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-02-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"639548"}}