{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:50:15Z","timestamp":1787017815497,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"\n Amoebiasis, a disease caused by\n Entamoeba histolytica<\/jats:italic>\n , is usually diagnosed by microscopic examination, which does not differentiate the morphologically identical species of the\n E. histolytica<\/jats:italic>\n \/\n E. dispar<\/jats:italic>\n complex. Furthermore, morphologically similar species such as\n Entamoeba hartmanni<\/jats:italic>\n contribute to misidentification. Therefore, there is a need for more sensitive and specific methods. This study standardized a multiplex real-time PCR system for\n E. histolytica<\/jats:italic>\n and\n E. dispar<\/jats:italic>\n and a single real-time PCR for\n E. hartmanni<\/jats:italic>\n . The multiplex protocol detected up to 0.0143\u2009pg of\n E. histolytica<\/jats:italic>\n DNA and 0.5156\u2009pg of\n E. dispar<\/jats:italic>\n DNA, and the average melting temperature (\n \n \n \n \n T<\/mml:mi>\n <\/mml:mrow>\n \n m<\/mml:mi>\n <\/mml:mrow>\n <\/mml:msub>\n <\/mml:mrow>\n <\/mml:math>\n ) was 73\u00b0C and 70\u00b0C, respectively. For\n E. hartmanni<\/jats:italic>\n , the\n \n \n \n \n T<\/mml:mi>\n <\/mml:mrow>\n \n m<\/mml:mi>\n <\/mml:mrow>\n <\/mml:msub>\n <\/mml:mrow>\n <\/mml:math>\n was 73\u00b0C and the amplification was successful down to 0.03\u2009fg of plasmid DNA. Negative controls and other intestinal parasites presented no amplification. Among the 48 samples tested,\n E. dispar<\/jats:italic>\n DNA was detected in 37; none exhibited\n E. histolytica<\/jats:italic>\n DNA and 11 were negative in the multiplex protocol. In 4 of these 11 samples, however,\n E. hartmanni<\/jats:italic>\n DNA was amplified. SYBR Green is demonstrated to be an interesting option and these combined PCR reactions can improve laboratory diagnosis of amoebiasis in developing countries.\n <\/jats:p>","DOI":"10.1155\/2014\/645084","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T16:05:15Z","timestamp":1392221115000},"page":"1-8","source":"Crossref","is-referenced-by-count":13,"title":["Differential Diagnosis of\n Entamoeba<\/i>\n spp. in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction"],"prefix":"10.1155","volume":"2014","author":[{"given":"Thiago dos Santos","family":"Gomes","sequence":"first","affiliation":[{"name":"Departamento de Doen\u00e7as Infecciosas e Parasit\u00e1rias, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Coimbra","family":"Garcia","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Prof. Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia de","family":"Souza Cunha","sequence":"additional","affiliation":[{"name":"Departamento de Doen\u00e7as Infecciosas e Parasit\u00e1rias, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4440-0546","authenticated-orcid":true,"given":"Heloisa","family":"Werneck de Macedo","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade Federal Fluminense, 24030-210 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Mauro","family":"Peralta","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Prof. Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina Helena Saramago","family":"Peralta","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade Federal Fluminense, 24030-210 Niter\u00f3i, RJ, Brazil"},{"name":"Departamento de Patologia, Hospital Universit\u00e1rio Ant\u00f4nio Pedro, Rua Marqu\u00eas do Paran\u00e1 303, Pr\u00e9dio Principal, 4\u00b0 andar, Centro, 24030-210 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00004-07"},{"key":"2","first-page":"97","volume":"72","year":"1997","journal-title":"Weekly Epidemiological 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