{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T14:47:06Z","timestamp":1775486826090,"version":"3.50.1"},"reference-count":40,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico-CNPq"},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro-FAPERJ"},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior-CAPES"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>The intracellular parasite<jats:italic>Toxoplasma gondii<\/jats:italic>can penetrate any warm-blooded animal cell. Conserved molecular assemblies of host cell plasma membranes should be involved in the parasite-host cell recognition. Lipid rafts are well-conserved membrane microdomains that contain high concentrations of cholesterol, sphingolipids, glycosylphosphatidylinositol, GPI-anchored proteins, and dually acylated proteins such as members of the Src family of tyrosine kinases. Disturbing lipid rafts of mouse peritoneal macrophages and epithelial cells of the lineage LLC-MK2 with methyl-beta cyclodextrin (M<jats:italic>\u03b2<\/jats:italic>CD) and filipin, which interfere with cholesterol or lidocaine, significantly inhibited internalization of<jats:italic>T. gondii<\/jats:italic>in both cell types, although adhesion remained unaffected in macrophages and decreased only in LLC-MK2 cells. Scanning and transmission electron microscopy confirmed these observations. Results are discussed in terms of the original role of macrophages as professional phagocytes versus the LLC-MK2 cell lineage originated from kidney epithelial cells.<\/jats:p>","DOI":"10.1155\/2014\/687835","type":"journal-article","created":{"date-parts":[[2014,3,9]],"date-time":"2014-03-09T17:02:18Z","timestamp":1394384538000},"page":"1-9","source":"Crossref","is-referenced-by-count":7,"title":["Disruption of Lipid Rafts Interferes with the Interaction of<i>Toxoplasma gondii<\/i>with Macrophages and Epithelial Cells"],"prefix":"10.1155","volume":"2014","author":[{"given":"Karla Dias","family":"Cruz","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Ultraestrutura Celular Hertha Meyer, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thayana 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