{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:52:03Z","timestamp":1768477923410,"version":"3.49.0"},"reference-count":43,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Jurinea dolomiaea<\/jats:italic>Boiss., family Compositae, is a medicinally important plant of alpine region. Its tuberous roots are used in various ailments in folk medicine. This study was undertaken to estimate total phenolic (TPC) and total flavonoid contents (TFC) and to determine anti-free radical potential by diverse<jats:italic>in vitro<\/jats:italic>antioxidant assays. Crude methanol extract (JDME) was fractionated into<jats:italic>n<\/jats:italic>-hexane (JDHE), chloroform (JDCE), ethyl acetate (JDEE),<jats:italic>n<\/jats:italic>-butanol (JDBE), and aqueous (JDAE) fractions. The results indicated that JDEE and JDCE constituted the highest amount of TFC (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn fontstyle=\"italic\">807<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">7.2<\/mml:mn><\/mml:math>\u2009mg rutin equivalent\/g sample) and TPC (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn fontstyle=\"italic\">757<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">9.4<\/mml:mn><\/mml:math>\u2009mg gallic acid equivalent\/g sample), respectively. Significant correlation of TFC with IC<jats:sub>50<\/jats:sub>values was recorded for<jats:sup>\u2022<\/jats:sup>OH (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:msup><mml:mrow><mml:mi>R<\/mml:mi><\/mml:mrow><mml:mrow><mml:mn fontstyle=\"italic\">2<\/mml:mn><\/mml:mrow><\/mml:msup><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.91<\/mml:mn><\/mml:math>), H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:msup><mml:mrow><mml:mi>R<\/mml:mi><\/mml:mrow><mml:mrow><mml:mn fontstyle=\"italic\">2<\/mml:mn><\/mml:mrow><\/mml:msup><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.82<\/mml:mn><\/mml:math>), and ABTS (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:msup><mml:mrow><mml:mi>R<\/mml:mi><\/mml:mrow><mml:mrow><mml:mn fontstyle=\"italic\">2<\/mml:mn><\/mml:mrow><\/mml:msup><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.82<\/mml:mn><\/mml:math>) assay. It could be made clear that JDEE was the most potent in antioxidant activity as compared to others, with generally lower IC<jats:sub>50<\/jats:sub>values for DPPH (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mn fontstyle=\"italic\">41.1<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">1.0<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), ABTS (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mn fontstyle=\"italic\">46.7<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.6<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M8\"><mml:mn fontstyle=\"italic\">42.2<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.9<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL),<jats:sup>\u2022<\/jats:sup>OH (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M9\"><mml:mn fontstyle=\"italic\">61.1<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">1.1<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL),<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M10\"><mml:mrow><mml:msup><mml:mrow><mml:msub><mml:mrow><mml:mtext>O<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mn fontstyle=\"italic\">2<\/mml:mn><\/mml:mrow><\/mml:msub><\/mml:mrow><mml:mrow><mml:mo>-<\/mml:mo><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M11\"><mml:mn fontstyle=\"italic\">152<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">1.1<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), and antilipid peroxidation (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M12\"><mml:mn fontstyle=\"italic\">54.3<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">1.6<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL). HPLC chromatogram of JDEE revealed the presence of catechin, caffeic acid, and rutin. The results indicated the antioxidant activities of<jats:italic>J. dolomiaea<\/jats:italic>roots and merit further investigations for their use in oxidative stress related disorders.<\/jats:p>","DOI":"10.1155\/2014\/726241","type":"journal-article","created":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T21:14:17Z","timestamp":1401311657000},"page":"1-10","source":"Crossref","is-referenced-by-count":31,"title":["Antioxidant Potential, DNA Protection, and HPLC-DAD Analysis of Neglected Medicinal<i>Jurinea dolomiaea<\/i>Roots"],"prefix":"10.1155","volume":"2014","author":[{"given":"Naseer Ali","family":"Shah","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Rashid","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiran","family":"Naz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mubarak Ali","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.08.026"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03033371"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2009.07.005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.06.027"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/S0308-8146(00)00171-0"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(97)00825-X"},{"issue":"4","key":"7","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1007\/s11746-998-0248-8","volume":"75","year":"1998","journal-title":"Journal of the American Oil Chemists' Society"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(98)00302-5"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.08.034"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2004.06.031"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1021\/jf030723c"},{"issue":"13","key":"12","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1002\/1097-0010(200010)80:13<1925::AID-JSFA714>3.0.CO;2-4","volume":"80","year":"2000","journal-title":"Journal of the Science of Food and Agriculture"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(00)00256-2"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1042\/BST0320156"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1177\/1082013202008003770"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2603"},{"key":"17","volume-title":"Indian medicinal plants","year":"1918"},{"issue":"10, article 5","key":"19","volume":"2009","year":"2009","journal-title":"Ethnobotanical Leaflets"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1186\/1746-4269-2-27"},{"key":"21","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.fct.2013.05.004","volume":"58","year":"2013","journal-title":"Food and Chemical Toxicology"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-13-276"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-13-140"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.03.120"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-12-221"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1021\/jf034108k"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1186\/1752-153X-6-12"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.3923\/pjbs.2009.447.450"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2004.06.018"},{"issue":"8","key":"30","first-page":"608","volume":"3","year":"2009","journal-title":"Journal of Medicinal Plants Research"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2005.10.005"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2126com"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.01.115"},{"issue":"4","key":"34","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3109\/10715769309145868","volume":"18","year":"1993","journal-title":"Free Radical Research Communications"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/S0367-326X(00)00300-2"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1080\/01926230290166724"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2004.02.008"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2005.09.019"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2004.06.042"},{"issue":"5","key":"40","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/0006-2952(88)90169-4","volume":"37","year":"1988","journal-title":"Biochemical Pharmacology"},{"issue":"11","key":"41","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1001\/archsurg.1997.01430350027005","volume":"132","year":"1997","journal-title":"Archives of Surgery"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1021\/jf000590k"},{"issue":"1","key":"43","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2174\/138620730101220118151526","volume":"1","year":"1998","journal-title":"Combinatorial Chemistry and High Throughput Screening"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1039\/b000080i"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/726241.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/726241.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/726241.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T02:59:09Z","timestamp":1649127549000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/726241\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":43,"alternative-id":["726241","726241"],"URL":"https:\/\/doi.org\/10.1155\/2014\/726241","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}