{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:38:36Z","timestamp":1740173916513,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,8,10]],"date-time":"2014-08-10T00:00:00Z","timestamp":1407628800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Scholarly Research Notices"],"published-print":{"date-parts":[[2014,8,10]]},"abstract":"<jats:p><jats:italic>Millettia macrophylla<\/jats:italic>is used traditionally to treat menopause related symptoms. This plant was shown to exhibit estrogenic effects<jats:italic>in vitro<\/jats:italic>on human embryonic kidney cells and<jats:italic>in vivo<\/jats:italic>on ovariectomized rats. The present study aimed at elucidating underlying mechanisms by which<jats:italic>M. macrophylla<\/jats:italic>induced its estrogenic effects. To accomplish our goal, kidney Hek293T cells transiently transfected with estrogen alpha or beta receptor expression plasmids were cotreated with a pure antiestrogen ICI 182,780 and the dichloromethane or methanol soluble fractions of<jats:italic>M. macrophylla.<\/jats:italic>To follow up, we cotreated ovariectomized rats with both extracts and ICI 182,780 for 3 days in the classical uterotrophic assay. Animals were then sacrificed and the uterine wet weight, total protein levels in uteri, uterine, and vaginal epithelial heights, and mammary gland were assessed.<jats:italic>In vitro,<\/jats:italic>the results suggested that the induction of the estrogenic activity by<jats:italic>M. macrophylla<\/jats:italic>is due to the binding of its secondary metabolites to ER<jats:italic>\u03b1<\/jats:italic>and ER<jats:italic>\u03b2<\/jats:italic>.<jats:italic>In vivo<\/jats:italic>, the cotreatment of extracts and ICI 182,780 significantly abrogated the biological responses induced by the extracts alone. Taken together, these results indicate that the active principles of<jats:italic>M. macrophylla<\/jats:italic>induce their beneficial effects on menopausal symptoms by activating the ERs.<\/jats:p>","DOI":"10.1155\/2014\/763781","type":"journal-article","created":{"date-parts":[[2014,8,10]],"date-time":"2014-08-10T21:08:50Z","timestamp":1407704930000},"page":"1-8","source":"Crossref","is-referenced-by-count":2,"title":["Elucidation of Underlying Mechanisms by Which<i>Millettia macrophylla<\/i>Benth Induces Its Estrogenic Activity"],"prefix":"10.1155","volume":"2014","author":[{"given":"St\u00e9phane","family":"Zingue","sequence":"first","affiliation":[{"name":"Laboratory of Physiology, Department of Life and Earth Sciences, Higher Teachers\u2019 Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon"},{"name":"Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal Beatrice","family":"Magne Nde","sequence":"additional","affiliation":[{"name":"MIMR-PHI Institute of Medical Research, Departments of Molecular and Translational Sciences, Monash University, 246 Clayton Road, Level 4, VIC 3168, Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0526-2475","authenticated-orcid":true,"given":"Colin","family":"Clyne","sequence":"additional","affiliation":[{"name":"MIMR-PHI Institute of Medical Research, Departments of Molecular and Translational Sciences, Monash University, 246 Clayton Road, Level 4, VIC 3168, Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dieudonn\u00e9","family":"Njamen","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, P.O. 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