{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T22:19:09Z","timestamp":1778797149961,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Extended-spectrum<jats:italic>\u03b2<\/jats:italic>-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains. In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including<jats:italic>Escherichia coli<\/jats:italic>(67.9%),<jats:italic>Klebsiella pneumoniae<\/jats:italic>(30.6%),<jats:italic>Klebsiella oxytoca<\/jats:italic>(0.5%),<jats:italic>Enterobacter aerogenes<\/jats:italic>(0.5%), and<jats:italic>Citrobacter freundii<\/jats:italic>(0.5%).<jats:italic>\u03b2<\/jats:italic>-lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. TEM enzymes were among the most prevalent types (40.9%) followed by CTX-M (37.3%) and SHV (23.3%). Among our sample of 193 ESBL-producing strains 99.0% were resistant to the fourth-generation cephalosporin cefepime. Of the 193 isolates 81.3% presented transferable plasmids harboring<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>ESBL<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>genes. Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences. This study reports a high diversity of genetic patterns. Ten clusters were found for<jats:italic>E. coli<\/jats:italic>isolates and five clusters for<jats:italic>K. pneumoniae<\/jats:italic>strains by means of ERIC analysis. In conclusion, in this country, the most prevalent type is still the TEM-type, but CTX-M is growing rapidly.<\/jats:p>","DOI":"10.1155\/2014\/782897","type":"journal-article","created":{"date-parts":[[2014,2,13]],"date-time":"2014-02-13T16:04:10Z","timestamp":1392307450000},"page":"1-6","source":"Crossref","is-referenced-by-count":34,"title":["Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8933-3984","authenticated-orcid":true,"given":"R\u00faben","family":"Fernandes","sequence":"first","affiliation":[{"name":"Ci\u00eancias Qu\u00edmicas e das Biomol\u00e9culas (CQB) e Centro de Investiga\u00e7\u00e3o em Sa\u00fade e Ambiente (CISA), Escola Superior de Tecnologia da Sa\u00fade do Porto (ESTSP), Instituto Polit\u00e9cnico do Porto (IPP), 4400-330 Porto, Portugal"},{"name":"CFBQ-Centro de Farmacologia e Biopatologia Qu\u00edmica (U38-FCT), Faculdade de Medicina do 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