{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:01:55Z","timestamp":1791424915758,"version":"4.3.3"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T00:00:00Z","timestamp":1395014400000},"content-version":"vor","delay-in-days":75,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>The chloroform extract of the stem bark of <jats:italic>Amburana cearensis<\/jats:italic> was chemically characterized and tested for antibacterial activity.The extract was analyzed by gas chromatography and mass spectrometry. The main compounds identified were 4\u2010methoxy\u20103\u2010methylphenol (76.7%), triciclene (3.9%), <jats:italic>\u03b1<\/jats:italic>\u2010pinene (1.0%), <jats:italic>\u03b2<\/jats:italic>\u2010pinene (2.2%), and 4\u2010hydroxybenzoic acid (3.1%). Preliminary antibacterial tests were carried out against species of distinct morphophysiological characteristics: <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Salmonella enterica<\/jats:italic> Serotype Typhimurium, <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, <jats:italic>Staphylococcus aureus<\/jats:italic>, <jats:italic>Listeria monocytogenes<\/jats:italic>, and <jats:italic>Bacillus cereus<\/jats:italic>. The minimum inhibitory concentration (MIC) was determinate in 96\u2010well microplates for the chloroform extract and an analogue of themain compound identified, which was purchased commercially.We have shown that plant\u2019s extract was only inhibitory (but not bactericidal) at the maximum concentration of 6900\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL against <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and <jats:italic>Bacillus cereus<\/jats:italic>. Conversely, the analogue 2\u2010methoxy\u20104\u2010methylphenol produced MICs ranging from215 to 431\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL against all bacterial species.New antibacterial assays conducted with such chemical compound against <jats:italic>Klebsiella pneumoniae<\/jats:italic> carbapenemase\u2010producing strains have shown similarMICresults and minimumbactericidal concentration (MBC) of 431\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL.We conclude that <jats:italic>A. cearensis<\/jats:italic> is a good source of methoxy\u2010methylphenol compounds,which could be screened for antibacterial activity againstmultiresistant bacteria fromdifferent species<\/jats:p>","DOI":"10.1155\/2014\/786586","type":"journal-article","created":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T21:03:23Z","timestamp":1395090203000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract of <i>Amburana cearensis<\/i> (Allem\u00e3o) A.C. Sm. against <i>Klebsiella pneumoniae<\/i> Carbapenemase\u2010Producing Strains"],"prefix":"10.1155","volume":"2014","author":[{"given":"Mirivaldo Barros","family":"S\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Taciana","family":"Ralph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle Cristina Oliveira","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9cio Souza","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isv\u00e2nia Maria Serafin","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabr\u00edcio Bezerra","family":"S\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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