{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T22:02:29Z","timestamp":1750456949287},"reference-count":19,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Canine cutaneous mast cell tumors (MCT) are the lethal skin tumors. The biological behavior of the MCT cells is quite varied and unpredictable. Almost MCT dogs usually require a rapid diagnosis and therapy. However, MCT diagnosis and prognosis are still dependent on histopathology which is rather inconvenient, time-consuming, painful, and harmful for some cases. Indeed, MCT can be easily accessible using fine-needle aspiration (FNA). In this study, our biopsy specimens were classified as low- and high-grade MCT based on the novel 2-tier histopathologic grading system. We have demonstrated the usage of fine-needle aspirated MCT cells (FNA-MCT cells) from these specimens as a primary cell source to study the distribution of CD117-immunocytochemistry (CD117-ICC) staining patterns and the frequency of internal tandem duplication- (ITD-) mutant exon-11 of<jats:italic>c-kit<\/jats:italic>. The result has substantially shown that there were three staining patterns identified in the cells. Only paranuclear pattern was significantly increased in the cells from high-grade MCT. Altogether, the ITD-mutant exon-11 was also detectable only in these cells. Therefore, the result has supported our hypothesis that there was an increased opportunity to observe a higher CD117-ICC staining pattern and exon-11 mutation in high-grade MCT; even these two parameters may not precisely indicate a histopathological grade.<\/jats:p>","DOI":"10.1155\/2014\/787498","type":"journal-article","created":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T21:03:51Z","timestamp":1392757431000},"page":"1-8","source":"Crossref","is-referenced-by-count":17,"title":["The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in<i>c-kit<\/i>Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells"],"prefix":"10.4061","volume":"2014","author":[{"given":"A.","family":"Sailasuta","sequence":"first","affiliation":[{"name":"STAR: Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Veterinary Oncology Unit, Small Animal 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