{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:51:02Z","timestamp":1791341462487,"version":"4.3.1"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2010\/05957-3"],"award-info":[{"award-number":["2010\/05957-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Aim.<\/jats:italic>This study sought to investigate the<jats:italic>in vitro<\/jats:italic>expression profile of high mobility group box 1 (HMGB1) in murine periodontal ligament fibroblasts (mPDL) stimulated with LPS or IL-1<jats:italic>\u03b2<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>during ligature- or LPS-induced periodontitis in rats.<jats:italic>Material and Methods.<\/jats:italic>For the<jats:italic>in vivo<\/jats:italic>study, 36 rats were divided into experimental and control groups, and biopsies were harvested at 7\u201330\u2009d following disease induction. Bone loss and inflammation were evaluated. HMGB1 expression was assessed by immunohistochemistry, qPCR, and Western blot.<jats:italic>Results.<\/jats:italic>Significant increases in mPDL HMGB1 mRNA occurred at 4, 8, and 12\u2009h with protein expression elevated by 24\u2009h. HMGB1 mRNA expression in gingival tissues was significantly increased at 15\u2009d in the LPS-PD model and at 7 and 15\u2009d in the ligature model. Immunohistochemical staining revealed a significant increase in the number of HMGB1-positive cells during the experimental periods.<jats:italic>Conclusion.<\/jats:italic>The results show that PDL cells produce HMGB1, which is increased and secreted extracellularly after inflammatory stimuli. In conclusion, this study demonstrates that HMGB1 may be associated with the onset and progression of periodontitis, suggesting that further studies should investigate the potential role of HMGB1 on periodontal tissue destruction.<\/jats:p>","DOI":"10.1155\/2014\/816320","type":"journal-article","created":{"date-parts":[[2014,2,20]],"date-time":"2014-02-20T16:03:30Z","timestamp":1392912210000},"page":"1-10","source":"Crossref","is-referenced-by-count":34,"title":["HMGB1 Localization during Experimental Periodontitis"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2756-5947","authenticated-orcid":true,"given":"Andressa Vilas Boas","family":"Nogueira","sequence":"first","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humait\u00e1, 1680, 2\u00b0 Andar, Centro, 14801-903 Araraquara, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Antonio","family":"Chaves de Souza","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humait\u00e1, 1680, 2\u00b0 Andar, Centro, 14801-903 Araraquara, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1110-6233","authenticated-orcid":true,"given":"Rafael Scaf","family":"de Molon","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humait\u00e1, 1680, 2\u00b0 Andar, Centro, 14801-903 Araraquara, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elyne","family":"da Silva Mariano Pereira","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humait\u00e1, 1680, 2\u00b0 Andar, Centro, 14801-903 Araraquara, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina Garcia","family":"de Aquino","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humait\u00e1, 1680, 2\u00b0 Andar, Centro, 14801-903 Araraquara, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William V.","family":"Giannobile","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, 1011 N. 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