{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:46:02Z","timestamp":1740170762105,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology","doi-asserted-by":"publisher","award":["SR\/S0\/BB-33\/2003","60(0106)\/13\/EMR-II"],"award-info":[{"award-number":["SR\/S0\/BB-33\/2003","60(0106)\/13\/EMR-II"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","award":["SR\/S0\/BB-33\/2003","60(0106)\/13\/EMR-II"],"award-info":[{"award-number":["SR\/S0\/BB-33\/2003","60(0106)\/13\/EMR-II"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Genomics"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Thermoanaerobacter indiensis<\/jats:italic>BSB-33 has been earlier shown to reduce Fe(III) and Cr(VI) anaerobically at 60\u00b0C optimally. Further, the Gram-positive thermophilic bacterium contains Cr(VI) reduction activity in both the membrane and cytoplasm. The soluble fraction prepared from<jats:italic>T. indiensis<\/jats:italic>cells grown at 60\u00b0C was found to contain the majority of Fe(III) reduction activity of the microorganism and produced four distinct bands in nondenaturing Fe(III) reductase activity gel. Proteins from each of these bands were partially purified by chromatography and identified by mass spectrometry (MS) with the help of<jats:italic>T. indiensis<\/jats:italic>proteome sequences. Two paralogous dihydrolipoamide dehydrogenases (LPDs), thioredoxin reductase (Trx), NADP(H)-nitrite reductase (Ntr), and thioredoxin disulfide reductase (Tdr) were determined to be responsible for Fe(III) reductase activity. Amino acid sequence and three-dimensional (3D) structural similarity analyses of the<jats:italic>T. indiensis<\/jats:italic>Fe(III) reductases were carried out with Cr(VI) reducing proteins from other bacteria. The two LPDs and Tdr showed very significant sequence and structural identity, respectively, with Cr(VI) reducing dihydrolipoamide dehydrogenase from<jats:italic>Thermus scotoductus<\/jats:italic>and thioredoxin disulfide reductase from<jats:italic>Desulfovibrio desulfuricans<\/jats:italic>. It appears that in addition to their iron reducing activity<jats:italic>T. indiensis<\/jats:italic>LPDs and Tdr are possibly involved in Cr(VI) reduction as well.<\/jats:p>","DOI":"10.1155\/2014\/850607","type":"journal-article","created":{"date-parts":[[2014,8,9]],"date-time":"2014-08-09T21:13:16Z","timestamp":1407618796000},"page":"1-9","source":"Crossref","is-referenced-by-count":2,"title":["Identification of Multiple Soluble Fe(III) Reductases in Gram-Positive Thermophilic Bacterium<i>Thermoanaerobacter indiensis<\/i>BSB-33"],"prefix":"10.1155","volume":"2014","author":[{"given":"Subrata","family":"Pal","sequence":"first","affiliation":[{"name":"Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700 032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"doi-asserted-by":"publisher","key":"1","DOI":"10.1016\/S0168-6445(03)00043-3"},{"doi-asserted-by":"publisher","key":"2","DOI":"10.1074\/jbc.M305291200"},{"doi-asserted-by":"publisher","key":"3","DOI":"10.1128\/JB.01750-12"},{"doi-asserted-by":"publisher","key":"4","DOI":"10.1111\/j.1574-6968.2009.01852.x"},{"doi-asserted-by":"publisher","key":"5","DOI":"10.1046\/j.1432-1033.2003.03957.x"},{"doi-asserted-by":"publisher","key":"6","DOI":"10.1128\/JB.183.15.4468-4476.2001"},{"issue":"1","key":"7","first-page":"3","volume":"7","year":"1994","journal-title":"BioMetals"},{"doi-asserted-by":"publisher","key":"8","DOI":"10.1111\/j.1365-2672.2009.04159.x"},{"doi-asserted-by":"publisher","key":"9","DOI":"10.1111\/j.1574-6968.2012.02555.x"},{"issue":"9","key":"10","doi-asserted-by":"crossref","first-page":"3188","DOI":"10.1128\/AEM.64.9.3188-3194.1998","volume":"64","year":"1998","journal-title":"Applied and Environmental Microbiology"},{"doi-asserted-by":"publisher","key":"11","DOI":"10.1093\/bioinformatics\/18.9.1250"},{"doi-asserted-by":"publisher","key":"12","DOI":"10.1093\/bioinformatics\/16.6.566"},{"doi-asserted-by":"publisher","key":"13","DOI":"10.1107\/S0907444904026460"},{"doi-asserted-by":"publisher","key":"14","DOI":"10.1002\/jcc.20084"},{"doi-asserted-by":"publisher","key":"15","DOI":"10.1021\/pr049816f"},{"doi-asserted-by":"publisher","key":"16","DOI":"10.1111\/j.1574-6968.2007.01063.x"},{"doi-asserted-by":"publisher","key":"17","DOI":"10.1110\/ps.12801"},{"doi-asserted-by":"publisher","key":"18","DOI":"10.1128\/JB.01035-09"},{"doi-asserted-by":"publisher","key":"19","DOI":"10.1038\/25720"},{"doi-asserted-by":"publisher","key":"20","DOI":"10.1128\/AEM.02463-10"},{"doi-asserted-by":"publisher","key":"21","DOI":"10.1007\/s00203-010-0622-4"},{"doi-asserted-by":"publisher","key":"22","DOI":"10.1016\/S0022-2836(02)00885-9"},{"doi-asserted-by":"publisher","key":"23","DOI":"10.1128\/JB.00197-09"},{"doi-asserted-by":"publisher","key":"24","DOI":"10.1016\/S0969-2126(01)00154-X"}],"container-title":["International Journal of Genomics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijg\/2014\/850607.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijg\/2014\/850607.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijg\/2014\/850607.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,23]],"date-time":"2020-08-23T02:24:15Z","timestamp":1598149455000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/ijg\/2014\/850607\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":24,"alternative-id":["850607","850607"],"URL":"https:\/\/doi.org\/10.1155\/2014\/850607","relation":{},"ISSN":["2314-436X","2314-4378"],"issn-type":[{"type":"print","value":"2314-436X"},{"type":"electronic","value":"2314-4378"}],"subject":[],"published":{"date-parts":[[2014]]}}}