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Injection of anti-LPS antibody and TLR4 blockade significantly (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mi>\u00a0<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mi>\u00a0<\/mml:mi><mml:mn>0.01<\/mml:mn><\/mml:math>) reduced the LPS dependent lowering amount of<jats:italic>GnRH<\/jats:italic>mRNA in the median eminence (ME). Moreover, blockade of TLR4 caused restoration of<jats:italic>LH-<jats:italic>\u03b2<\/jats:italic><\/jats:italic>transcription in the anterior pituitary decreased by the immune stress. However, there was no effect of this treatment on reduced LH release. The results of our study showed that the blockade of TLR4 receptor in the hypothalamus is not sufficient to unblock the release of LH suppressed by the immune\/inflammatory challenges. This suggests that during inflammation the LH secretion could be inhibited directly at the pituitary level by peripheral factors such as proinflammatory cytokines and circulating endotoxin as well.<\/jats:p>","DOI":"10.1155\/2014\/867170","type":"journal-article","created":{"date-parts":[[2014,2,11]],"date-time":"2014-02-11T21:02:26Z","timestamp":1392152546000},"page":"1-10","source":"Crossref","is-referenced-by-count":31,"title":["Effects of Central Injection of Anti-LPS Antibody and Blockade of TLR4 on GnRH\/LH Secretion during Immunological Stress in Anestrous Ewes"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3389-3912","authenticated-orcid":true,"given":"Karolina","family":"Haziak","sequence":"first","affiliation":[{"name":"The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jab\u0142onna, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej 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