{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:30:57Z","timestamp":1787614257134,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004700","name":"Taipei Medical University","doi-asserted-by":"publisher","award":["TMU98-AE1-B18"],"award-info":[{"award-number":["TMU98-AE1-B18"]}],"id":[{"id":"10.13039\/501100004700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Butylidenephthalide (Bdph, 30~300\u2009<jats:italic>\u03bc<\/jats:italic>M), a constituent of<jats:italic>Ligusticum chuanxiong<\/jats:italic>Hort., significantly enhanced tension in isolated guinea-pig trachea. In this study, we investigate the mechanism(s) of Bdph-induced contraction in the tissue. Isolated trachea was bathed in 5\u2009mL of Krebs solution containing indomethacin (3\u2009<jats:italic>\u03bc<\/jats:italic>M), and its tension changes were isometrically recorded. Cromakalim (3\u2009<jats:italic>\u03bc<\/jats:italic>M), an ATP-dependent K<jats:sup>+<\/jats:sup>channel opener, significantly antagonized the Bdph-induced enhancement of baseline tension. Bdph (300\u2009<jats:italic>\u03bc<\/jats:italic>M) also significantly antagonized cromakalim-induced relaxation. Bdph (300\u2009<jats:italic>\u03bc<\/jats:italic>M) did not significantly influence the antagonistic effects of glibenclamide (GBC, 1\u2009<jats:italic>\u03bc<\/jats:italic>M) and tetraethylammonium (TEA, 8\u2009mM) against the cromakalim-induced relaxation. However, Bdph (300\u2009<jats:italic>\u03bc<\/jats:italic>M) and 4-aminopiridine (4-AP, 5\u2009mM), a blocker of K<jats:sub><jats:italic>v<\/jats:italic><\/jats:sub>1 family of K<jats:sup>+<\/jats:sup>channels, in combination significantly rightward shifted the log concentration-relaxation curve of cromakalim. The antagonistic effect of the combination almost equals the sum of the individual effects of Bdph and 4-AP, suggesting that the antagonistic mechanism of Bdph may be similar to that of 4-AP. All calcium channel blockers influenced neither the baseline tension nor antagonistic effect of Bdph against cromakalim. In conclusion, Bdph may be similar to 4-AP, a blocker of K<jats:sub><jats:italic>v<\/jats:italic><\/jats:sub>1 family of K<jats:sup>+<\/jats:sup>channels, to enhance the baseline tension of guinea-pig trachea.<\/jats:p>","DOI":"10.1155\/2014\/875230","type":"journal-article","created":{"date-parts":[[2014,7,10]],"date-time":"2014-07-10T17:16:05Z","timestamp":1405012565000},"page":"1-7","source":"Crossref","is-referenced-by-count":6,"title":["Butylidenephthalide Blocks Potassium Channels and Enhances Basal Tension in Isolated Guinea-Pig Trachea"],"prefix":"10.1155","volume":"2014","author":[{"given":"Hsin-Te","family":"Hsu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, Taipei Medical University Hospital, 252 Wu-Hsing Street, Taipei 110, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Lan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Respiratory Therapy, College of Medicine, Taipei Medical University, 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