{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T11:54:14Z","timestamp":1753358054402,"version":"3.40.4"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Mycoplasma agalactiae<\/jats:italic>and<jats:italic>Mycoplasma bovis<\/jats:italic>both are responsible for respiratory conditions in sheep and goats.<jats:italic>M. agalactiae<\/jats:italic>is a major pathogen of sheep and goats and accounts for almost 90% of outbreaks of contagious agalactia syndrome in goats and almost 100% in sheep. On the basis of clinical signs and cultural, morphological, and biochemical characterization it is almost impossible to differentiate between both the species. Moreover, due to presence of genomic and proteomic similarity most of the time routine diagnostic tests fail to differentiate between them. Hence the present study was conducted to find out the protein profile of isolates of both the species by SDS-PAGE and to find out the cross-reacting as well as differentiating immunogenic proteins by Immunoblotting, which can be of immunoprophylactic as well as diagnostic values. The study revealed 6-7 major immunogenic cross-reactive proteins with the presence of two important non-cross-reacting species specific polypeptides particularly 25.50 and 24.54\u2009kDa in<jats:italic>M. agalactiae<\/jats:italic>and<jats:italic>M. bovis<\/jats:italic>, respectively, that might be of diagnostic values.<\/jats:p>","DOI":"10.1155\/2014\/892421","type":"journal-article","created":{"date-parts":[[2014,4,3]],"date-time":"2014-04-03T21:10:06Z","timestamp":1396559406000},"page":"1-6","source":"Crossref","is-referenced-by-count":3,"title":["Electrophoretic Analysis of Indian Isolates of<i>Mycoplasma agalactiae<\/i>and<i>Mycoplasma bovis<\/i>by SDS-PAGE and Immunoblotting"],"prefix":"10.4061","volume":"2014","author":[{"given":"Amit","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Microbiology, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwa Vidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura 281001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. C.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"National Referral Laboratory on Mycoplasma, Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izzatnagar 243122, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. P.","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izzatnagar 243122, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jai","family":"Sunder","sequence":"additional","affiliation":[{"name":"Divisionof Animal Science, Central Agricultural Research Institute, Port Blair, A & N Islands 744101, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"4","key":"1","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1128\/MMBR.62.4.1094-1156.1998","volume":"62","year":"1998","journal-title":"Microbiology and Molecular Biology Reviews"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3923\/ajava.2011.537.546"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3923\/ajava.2012.149.157"},{"key":"4","first-page":"699","volume":"6","year":"1987","journal-title":"Revue Scientifique et Technique. Office International des Epizooties"},{"issue":"2","key":"5","first-page":"161","volume":"23","year":"2002","journal-title":"Indian Journal of Comparative Microbiology Immunology and Infectious Diseases"},{"year":"1987","series-title":"Edited by: M. Lambert and G. Jones","key":"6"},{"year":"1996","series-title":"Edited by: J. Frey and K. Sarris","key":"7"},{"issue":"3","key":"8","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1017\/S0022172400065050","volume":"93","year":"1984","journal-title":"Journal of Hygiene"},{"first-page":"100","volume-title":"An economic appraisal of the acute form of contagious agalactia in the transhumant sheep and goats in Greece","year":"1996","key":"9"},{"issue":"3","key":"11","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1099\/00207713-26-3-323","volume":"26","year":"1976","journal-title":"International Journal of Systematic Bacteriology"},{"first-page":"27","volume-title":"Toxonomic relationship between M. bovis and M. agalactiae","year":"1996","key":"12"},{"issue":"3","key":"13","doi-asserted-by":"crossref","first-page":"433","DOI":"10.2460\/ajvr.1983.44.03.433","volume":"44","year":"1983","journal-title":"American Journal of Veterinary Research"},{"issue":"1-2","key":"14","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0378-1135(95)00200-6","volume":"50","year":"1996","journal-title":"Veterinary Microbiology"},{"issue":"1","key":"15","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","year":"1951","journal-title":"The Journal of Biological Chemistry"},{"issue":"6","key":"16","first-page":"542","volume":"66","year":"1996","journal-title":"Indian Journal of Animal Sciences"},{"issue":"5","key":"19","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1128\/AEM.22.5.756-759.1971","volume":"22","year":"1971","journal-title":"Applied Microbiology"},{"key":"20","first-page":"1037","volume":"66","year":"1992","journal-title":"Indian Journal of Animal Sciences"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"issue":"3-4","key":"22","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0165-2427(88)90111-0","volume":"19","year":"1988","journal-title":"Veterinary Immunology and Immunopathology"},{"issue":"9","key":"23","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","volume":"76","year":"1979","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"1","key":"24","first-page":"271","volume":"16","year":"2010","journal-title":"Indian Journal of Small Ruminants"},{"first-page":"91","volume-title":"PCR on16SrRNA gene, REA and SDS-PAGE patterns of M. agalactiae isolates","year":"1996","key":"25"},{"issue":"4","key":"26","first-page":"246","volume":"39","year":"1992","journal-title":"Journal of Veterinary Medicine B"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(94)90119-8"},{"issue":"1","key":"29","first-page":"181","volume":"133","year":"1987","journal-title":"Journal of General Microbiology"},{"issue":"1","key":"30","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3923\/jm.2014.59.65","volume":"9","year":"2014","journal-title":"Research Journal of Microbiology"},{"issue":"1","key":"32","first-page":"29","volume":"34","year":"2013","journal-title":"Indian Journal of Comparative Microbiology and Infectious Diseases"},{"issue":"1","key":"33","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1099\/00221287-75-1-135","volume":"75","year":"1973","journal-title":"Journal of General Microbiology"}],"container-title":["Veterinary Medicine International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/vmi\/2014\/892421.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/vmi\/2014\/892421.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/vmi\/2014\/892421.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T07:32:38Z","timestamp":1746171158000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/vmi\/2014\/892421\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":28,"alternative-id":["892421","892421"],"URL":"https:\/\/doi.org\/10.1155\/2014\/892421","relation":{},"ISSN":["2090-8113","2042-0048"],"issn-type":[{"type":"print","value":"2090-8113"},{"type":"electronic","value":"2042-0048"}],"subject":[],"published":{"date-parts":[[2014]]}}}