{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:22:13Z","timestamp":1762521733285},"reference-count":20,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Creative Research Groups of Gansu Province","award":["1210RJIA006","2012BAK11B05","20123A299"],"award-info":[{"award-number":["1210RJIA006","2012BAK11B05","20123A299"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Clonorchis sinensis<\/jats:italic>and<jats:italic>Opisthorchis viverrini<\/jats:italic>are both important fish-borne pathogens, causing serious public health problem in Asia. The present study developed an assay integrating real-time PCR and high resolution melting (HRM) analysis for the specific detection and rapid identification of<jats:italic>C. sinensis<\/jats:italic>and<jats:italic>O. viverrini<\/jats:italic>. Primers targeting COX1 gene were highly specific for these liver flukes, as evidenced by the negative amplification of closely related trematodes. Assays using genomic DNA extracted from the two flukes yielded specific amplification and their identity was confirmed by sequencing, having the accuracy of 100% in reference to conventional methods. The assay was proved to be highly sensitive with a detection limit below 1 pg of purified genomic DNA, 5 EPG, or 1 metacercaria of<jats:italic>C. sinensis<\/jats:italic>. Moreover,<jats:italic>C. sinensis<\/jats:italic>and<jats:italic>O. viverrini<\/jats:italic>were able to be differentiated by their HRM profiles. The method can reduce labor of microscopic examination and the contamination of agarose electrophoresis. Moreover, it can differentiate these two flukes which are difficult to be distinguished using other methods. The established method provides an alternative tool for rapid, simple, and duplex detection of<jats:italic>C. sinensis<\/jats:italic>and<jats:italic>O. viverrini<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2014\/893981","type":"journal-article","created":{"date-parts":[[2014,10,19]],"date-time":"2014-10-19T17:05:28Z","timestamp":1413738328000},"page":"1-6","source":"Crossref","is-referenced-by-count":14,"title":["Rapid Detection and Differentiation of<i>Clonorchis sinensis<\/i>and<i>Opisthorchis viverrini<\/i>Using Real-Time PCR and High Resolution Melting Analysis"],"prefix":"10.1155","volume":"2014","author":[{"given":"Xian-Quan","family":"Cai","sequence":"first","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, China"},{"name":"Technical Center, Zhongshan Entry-Exit Inspection and 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