{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:03:21Z","timestamp":1788851001332,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007398","name":"Secretaria de Estado de Sa\u00fade de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007398","id-type":"DOI","asserted-by":"publisher"}]},{"name":"N\u00fa\u00e7leo de A\u00e7 \u00f5 es e Pesquisa em Apoio Diagn\u00f3stico"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic>is the main infectious cause of human posterior retinochoroiditis, the most frequent clinical manifestation of congenital toxoplasmosis. This investigation was performed after neonatal screening to identify biomarkers of immunity associated with immunopathological features of the disease by flow cytometry. The study included infected infants without NRL and with retinochoroidal lesions (ARL, ACRL, and CRL) as well as noninfected individuals (NI). Our data demonstrated that leukocytosis, with increased monocytes and lymphocytes, was a relevant hematological biomarker of ARL. Immunophenotypic analysis also revealed expansion of CD14<jats:sup>+<\/jats:sup>CD16<jats:sup>+<\/jats:sup>HLA-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msup><mml:mrow><mml:mtext>DR<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>high<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>monocytes and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mtext>CD<\/mml:mtext><mml:msup><mml:mrow><mml:mn>56<\/mml:mn><\/mml:mrow><mml:mrow><mml:mtext>dim<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>cytotoxic NK-cells in ARL. Moreover, augmented TCR<jats:italic>\u03b3<\/jats:italic><jats:italic>\u03b4<\/jats:italic><jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T-cell counts were apparently good biomarkers of morbidity. Biomarker network analysis revealed that complex and intricated networks underscored the negative correlation of monocytes with NK- and B-cells in NRL. The remarkable lack of connections involving B-cells and a relevant shift of NK-cell connections from B-cells toward T-cells observed in ARL were outstanding. A tightly connected biomarker network was observed in CRL, with relevant connections of NK- and CD8<jats:sup>+<\/jats:sup>T-cells with a broad range of cell subsets. Our findings add novel elements to the current knowledge on the innate and adaptive immune responses in congenital toxoplasmosis.<\/jats:p>","DOI":"10.1155\/2014\/910621","type":"journal-article","created":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T21:17:27Z","timestamp":1411075047000},"page":"1-13","source":"Crossref","is-referenced-by-count":43,"title":["Biomarker Analysis Revealed Distinct Profiles of Innate and Adaptive Immunity in Infants with Ocular Lesions of Congenital Toxoplasmosis"],"prefix":"10.1155","volume":"2014","author":[{"given":"Anderson Silva","family":"Machado","sequence":"first","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Presidente Ant\u00f4nio Carlos, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina Aguiar Vasconcelos","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, 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