{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:20:37Z","timestamp":1787235637875,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100010679","name":"Pasteur Institute of Iran","doi-asserted-by":"crossref","award":["605"],"award-info":[{"award-number":["605"]}],"id":[{"id":"10.13039\/501100010679","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>\n                    Cutaneous leishmaniasis has various phenotypic aspects consisting of polymorphic amastigotes with different genetic ranges. Samples were collected from suspected patients of Khuzestan province. Prepared smears were stained, scaled, and measured using ocular micrometer. The Cyt\n                    <jats:italic>b<\/jats:italic>\n                    , ITS-rDNA, and microsatellite genes of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    were amplified and\n                    <jats:italic>Leishmania<\/jats:italic>\n                    species were identified by molecular analyses. Of 150 examined suspected patients, 102 were identified to\n                    <jats:italic>Leishmania<\/jats:italic>\n                    species (90\n                    <jats:italic>L. major<\/jats:italic>\n                    , nine\n                    <jats:italic>L. tropica<\/jats:italic>\n                    , and three unidentified). The amastigotes of 90\n                    <jats:italic>L. major<\/jats:italic>\n                    had regular and different irregular shapes within three clinical lesions with no and\/or low genetic diversity. Three haplotypes of Cyt\n                    <jats:italic>b<\/jats:italic>\n                    of\n                    <jats:italic>L. major<\/jats:italic>\n                    were found but no variation was observed using ITS-rDNA gene. Interesting findings were that all nine\n                    <jats:italic>L. tropica<\/jats:italic>\n                    had regular amastigote shapes with more genetic variations, also a patient which had coinfection of\n                    <jats:italic>L. major<\/jats:italic>\n                    ,\n                    <jats:italic>L. tropica<\/jats:italic>\n                    , and\n                    <jats:italic>Crithidia.<\/jats:italic>\n                    At least two\n                    <jats:italic>L. major<\/jats:italic>\n                    and\n                    <jats:italic>L. tropica<\/jats:italic>\n                    were identified in suspected patients of the regions. Different irregular amastigotes\u2019 shapes of\n                    <jats:italic>L. major<\/jats:italic>\n                    can be explained by various reservoir hosts and vectors. In contrast, more molecular variations in\n                    <jats:italic>L. tropica<\/jats:italic>\n                    could be justified by genetic characters. Unidentified\n                    <jats:italic>Leishmania<\/jats:italic>\n                    could be mixed pathogens or nonpathogens with mammals\u2019\n                    <jats:italic>Leishmania<\/jats:italic>\n                    or\n                    <jats:italic>Crithidia<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1155\/2014\/913510","type":"journal-article","created":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T21:46:10Z","timestamp":1410903970000},"page":"1-13","source":"Crossref","is-referenced-by-count":27,"title":["The Associations of\n                    <i>Leishmania major<\/i>\n                    and\n                    <i>Leishmania tropica<\/i>\n                    Aspects by Focusing Their Morphological and Molecular Features on Clinical Appearances in Khuzestan Province, Iran"],"prefix":"10.1155","volume":"2014","author":[{"given":"Adel","family":"Spotin","sequence":"first","affiliation":[{"name":"Parasitology Department, Medical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran"},{"name":"Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, 69 Pasteur Avenue, 1316943551 Tehran, Iran"},{"name":"Parasitology Department, Medical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soheila","family":"Rouhani","sequence":"additional","affiliation":[{"name":"Parasitology Department, Medical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parviz","family":"Parvizi","sequence":"additional","affiliation":[{"name":"Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, 69 Pasteur Avenue, 1316943551 Tehran, 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