{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T21:56:12Z","timestamp":1696370172363},"reference-count":49,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. To evaluate the dose effect of vitamin K<jats:sub>3<\/jats:sub>on wound healing mechanisms.<jats:italic>Methods<\/jats:italic>. Conjunctival fibroblasts were incubated for 24 hours. An artificial wound was made and the cells were incubated with fresh medium plus doses of vitamin K<jats:sub>3<\/jats:sub>to be tested. Wound repair was monitored at 0, 18, 24, and 48 hours. Proliferation was measured in actively dividing cells by [<jats:sup>3<\/jats:sup>H]thymidine uptake. Six different groups were tested: group 1\/no drugs added, group 2\/ethanol 0.1%, group 3\/vitamin K<jats:sub>3<\/jats:sub>1\u2009mg\/L, group 4\/vitamin K<jats:sub>3<\/jats:sub>2\u2009mg\/L, group 5\/vitamin K<jats:sub>3<\/jats:sub>4\u2009mg\/L, and group 6\/vitamin K<jats:sub>3<\/jats:sub>6\u2009mg\/L. Each experiment was carried out in triplicate and 4 times.<jats:italic>Results<\/jats:italic>. There were no differences among groups at the initial time.<jats:italic>In vitro<\/jats:italic>wound repair was slower in groups 4, 5, and 6. There were no differences between control and ethanol groups and between control and vitamin K<jats:sub>3<\/jats:sub>1 mg\/L groups. Fibroblast mitogenic activity was statistically decreased in all vitamin K groups; statistical differences were found among vitamin K<jats:sub>3<\/jats:sub>1\u2009mg\/mL and higher doses too. In groups 5 and 6, cellular toxicity was presented.<jats:italic>Conclusions<\/jats:italic>. Vitamin K<jats:sub>3<\/jats:sub>is able to inhibit fibroblast proliferation. Vitamin K<jats:sub>3<\/jats:sub>2\u2009mg\/L or higher doses inhibit wound healing repair, exhibiting cellular toxicity at 4 and 6\u2009mg\/L.<\/jats:p>","DOI":"10.1155\/2014\/916713","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T21:24:47Z","timestamp":1389216287000},"page":"1-5","source":"Crossref","is-referenced-by-count":1,"title":["<i>In Vitro<\/i>Vitamin K<sub>3<\/sub>Effect on Conjunctival Fibroblast Migration and Proliferation"],"prefix":"10.1155","volume":"2014","author":[{"given":"I.","family":"Pinilla","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Lozano Blesa University Hospital, C\/San Juan Bosco 15, 50009 Zaragoza, Spain"},{"name":"Aragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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