{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:28:55Z","timestamp":1740169735970,"version":"3.37.3"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>We have used a previously published computer model of the rat cardiac ventricular myocyte to investigate the effect of changing the distribution of Ca<jats:sup>2+<\/jats:sup>efflux pathways (SERCA, Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup>exchange, and sarcolemmal Ca<jats:sup>2+<\/jats:sup>ATPase) between the dyad and bulk cytoplasm and the effect of adding exogenous Ca<jats:sup>2+<\/jats:sup>buffers (BAPTA or EGTA), which are used experimentally to differentially buffer Ca<jats:sup>2+<\/jats:sup>in the dyad and bulk cytoplasm, on cellular Ca<jats:sup>2+<\/jats:sup>cycling. Increasing the dyadic fraction of a particular Ca<jats:sup>2+<\/jats:sup>efflux pathway increases the amount of Ca<jats:sup>2+<\/jats:sup>removed by that pathway, with corresponding changes in Ca<jats:sup>2+<\/jats:sup>efflux from the bulk cytoplasm. The magnitude of these effects varies with the proportion of the total Ca<jats:sup>2+<\/jats:sup>removed from the cytoplasm by that pathway. Differences in the response to EGTA and BAPTA, including changes in Ca<jats:sup>2+<\/jats:sup>-dependent inactivation of the L-type Ca<jats:sup>2+<\/jats:sup>current, resulted from the buffers acting as slow and fast \u201cshuttles,\u201d respectively, removing Ca<jats:sup>2+<\/jats:sup>from the dyadic space. The data suggest that complex changes in dyadic Ca<jats:sup>2+<\/jats:sup>and cellular Ca<jats:sup>2+<\/jats:sup>cycling occur as a result of changes in the location of Ca<jats:sup>2+<\/jats:sup>removal pathways or the presence of exogenous Ca<jats:sup>2+<\/jats:sup>buffers, although changing the distribution of Ca<jats:sup>2+<\/jats:sup>efflux pathways has relatively small effects on the systolic Ca<jats:sup>2+<\/jats:sup>transient.<\/jats:p>","DOI":"10.1155\/2014\/920208","type":"journal-article","created":{"date-parts":[[2014,5,29]],"date-time":"2014-05-29T17:14:35Z","timestamp":1401383675000},"page":"1-12","source":"Crossref","is-referenced-by-count":6,"title":["Effect of Ca<sup>2+<\/sup>Efflux Pathway Distribution and Exogenous Ca<sup>2+<\/sup>Buffers on Intracellular Ca<sup>2+<\/sup>Dynamics in the Rat Ventricular Myocyte: A Simulation Study"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8198-1215","authenticated-orcid":true,"given":"Michal","family":"P\u00e1sek","sequence":"first","affiliation":[{"name":"Institute of Thermomechanics, Branch Brno, Academy of Science of the Czech Republic, Technick\u00e1 2, 61669 Brno, Czech Republic"},{"name":"Department of Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u0159\u00ed","family":"\u0160imurda","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clive H.","family":"Orchard","sequence":"additional","affiliation":[{"name":"School of Physiology and Pharmacology, University of Bristol, Bristol BS8 1TD, 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