{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:12:33Z","timestamp":1777468353894,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"National Science Centre in Poland","award":["2011\/02\/A\/NZ5\/00321"],"award-info":[{"award-number":["2011\/02\/A\/NZ5\/00321"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Enhanced expression of cell cycle inhibitor<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mtext>p<\/mml:mtext><mml:msup><mml:mrow><mml:mtext>27<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>kip1<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>suppresses cell proliferation. Ursodeoxycholic acid (UDCA) delays progression of primary biliary cirrhosis (PBC) but its effect on<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mtext>p<\/mml:mtext><mml:msup><mml:mrow><mml:mtext>27<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>kip1<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>expression is uncertain.<jats:italic>Aims<\/jats:italic>. To analyze the expression of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mtext>p<\/mml:mtext><mml:msup><mml:mrow><mml:mtext>27<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>kip1<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>and its transcription modulator FoxO1 in patients with PBC, and to assess the impact of UDCA on this pathway.<jats:italic>Materials and Methods<\/jats:italic>. The examined human tissue included explanted livers from patients with cirrhotic PBC (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>23<\/mml:mn><\/mml:math>), primary sclerosing cholangitis (PSC;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>9<\/mml:mn><\/mml:math>), alcoholic liver disease (ALD;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>9<\/mml:mn><\/mml:math>), and routine liver biopsies from patients with non-cirrhotic PBC (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M8\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>26<\/mml:mn><\/mml:math>). Healthy liver samples served as controls (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M9\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>19<\/mml:mn><\/mml:math>). Livers of FoxO-deficient mice were also studied. mRNA and protein expressions were analyzed by real-time PCR and Western blot.<jats:italic>Results<\/jats:italic>.<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M10\"><mml:mtext>p<\/mml:mtext><mml:msup><mml:mrow><mml:mtext>27<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>kip1<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>expression was increased in cirrhotic and non-cirrhotic PBC. FoxO1 mRNA levels were increased in PBC (8.5-fold increase versus controls). FoxO1 protein expression in PBC was comparable to controls, but it was decreased in patients with PSC and ALD (63% and 70% reduction, respectively; both<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M11\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>versus control). UDCA-treated non-cirrhotic patients with PBC showed decreased expression of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M12\"><mml:mtext>p<\/mml:mtext><mml:msup><mml:mrow><mml:mtext>27<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>kip1<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>mRNA.<jats:italic>Conclusion<\/jats:italic>. PBC progression is characterized by a FoxO1-independent increase of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M13\"><mml:mtext>p<\/mml:mtext><mml:msup><mml:mrow><mml:mtext>27<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>kip1<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>expression. In early PBC, UDCA may enhance liver regeneration via<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M14\"><mml:mtext>p<\/mml:mtext><mml:msup><mml:mrow><mml:mtext>27<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>kip1<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>-dependent mechanism.<\/jats:p>","DOI":"10.1155\/2014\/921285","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:05:25Z","timestamp":1391616325000},"page":"1-8","source":"Crossref","is-referenced-by-count":6,"title":["Ursodeoxycholic Acid Influences the Expression of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mtext>p<\/mml:mtext><mml:msup><mml:mrow><mml:mtext>27<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>kip1<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:math>but Not FoxO1 in Patients with Non-Cirrhotic Primary Biliary Cirrhosis"],"prefix":"10.1155","volume":"2014","author":[{"given":"Malgorzata","family":"Milkiewicz","sequence":"first","affiliation":[{"name":"Medical Biology Laboratory, Pomeranian Medical University, Aleja Powstancow Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justyna","family":"Kopyci\u0144ska","sequence":"additional","affiliation":[{"name":"Medical Biology Laboratory, Pomeranian Medical University, Aleja Powstancow Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Kempi\u0144ska-Podhorodecka","sequence":"additional","affiliation":[{"name":"Medical Biology Laboratory, Pomeranian Medical University, Aleja Powstancow Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tara","family":"Haas","sequence":"additional","affiliation":[{"name":"Angiogenesis Research Group, Faculty of Health, York University, Toronto, 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