{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:43:53Z","timestamp":1788871433826,"version":"build-2803163510"},"reference-count":67,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Ministry of Science and Technology"},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)"},{"name":"Financiadora de Estudos e Projetos (FINEP)"},{"name":"Funda\u00e7\u00e3o de Tecnologia do Acre (FUNTAC\/FDCT)"},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005292","name":"Instituto Nacional para Pesquisa Translacional em Sa\u00fade e Ambiente na Regi\u00e3o Amaz\u00f4nica","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005292","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004933","name":"Instituto Nacional para Pesquisa em Toxinas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004933","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Secretary of Development of Rondonia State"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>\n                    Toxins purified from the venom of spiders have high potential to be studied pharmacologically and biochemically. These biomolecules may have biotechnological and therapeutic applications. This study aimed to evaluate the protein content of\n                    <jats:italic>Parawixia bistriata<\/jats:italic>\n                    venom and functionally characterize its proteins that have potential for biotechnological applications. The crude venom showed no phospholipase, hemorrhagic, or anti-Leishmania activities attesting to low genotoxicity and discrete antifungal activity for\n                    <jats:italic>C. albicans<\/jats:italic>\n                    . However the following activities were observed: anticoagulation, edema, myotoxicity and proteolysis on casein, azo-collagen, and fibrinogen. The chromatographic and electrophoretic profiles of the proteins revealed a predominance of acidic, neutral, and polar proteins, highlighting the presence of proteins with high molecular masses. Five fractions were collected using cation exchange chromatography, with the P4 fraction standing out as that of the highest purity. All fractions showed proteolytic activity. The crude venom and fractions P1, P2, and P3 showed larvicidal effects on\n                    <jats:italic>A. aegypti<\/jats:italic>\n                    . Fraction P4 showed the presence of a possible metalloprotease (60\u2009kDa) that has high proteolytic activity on azo-collagen and was inhibited by EDTA. The results presented in this study demonstrate the presence of proteins in the venom of\n                    <jats:italic>P. bistriata<\/jats:italic>\n                    with potential for biotechnological applications.\n                  <\/jats:p>","DOI":"10.1155\/2014\/950538","type":"journal-article","created":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T17:19:36Z","timestamp":1399483176000},"page":"1-13","source":"Crossref","is-referenced-by-count":3,"title":["Biochemical and Functional Characterization of\n                    <i>Parawixia bistriata<\/i>\n                    Spider Venom with Potential Proteolytic and Larvicidal Activities"],"prefix":"10.1155","volume":"2014","author":[{"given":"Gizeli S.","family":"Gimenez","sequence":"first","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas a Sa\u00fade, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, Universidade Federal de Rond\u00f4nia, UNIR, 76812-245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Coutinho-Neto","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas a Sa\u00fade, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, Universidade Federal de Rond\u00f4nia, UNIR, 76812-245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson M.","family":"Kayano","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas a Sa\u00fade, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, Universidade Federal de Rond\u00f4nia, UNIR, 76812-245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Sim\u00f5es-Silva","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas a Sa\u00fade, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, Universidade Federal de Rond\u00f4nia, UNIR, 76812-245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances","family":"Trindade","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Entomologia M\u00e9dica, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Laborat\u00f3rio de Bioecologia de Insetos, Departamento de Biologia, UNIR, 76812-245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"de Almeida e Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Entomologia M\u00e9dica, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Laborat\u00f3rio de Bioecologia de Insetos, Departamento de Biologia, UNIR, 76812-245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Marcussi","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Universidades Federal de Lavras, UFLA, 37200-000 Lavras, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saulo L.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Biotecnologia e Engenharia de Bioprocessos, Universidade Federal de S\u00e3o Jo\u00e3o del Rei, UFSJ, Campus Altoparaopeba, 36420-000 Ouro Branco, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla F. 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