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The mutant Mb\u0394p27-p55 was significantly more attenuated in nude mice than its parental strain but more virulent than BCG Pasteur. Challenge experiments in mice and guinea pigs using<jats:italic>M. bovis<\/jats:italic>or<jats:italic>M. tuberculosis<\/jats:italic>strains showed similar protection conferred by Mb\u0394p27-p55 mutant than BCG in terms of pathology and bacterial loads in spleen but lower protection than BCG in lungs. 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