{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:35:55Z","timestamp":1770993355445,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["FAPESP 2011\/03862-8"],"award-info":[{"award-number":["FAPESP 2011\/03862-8"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["FAPESP 2011\/03862-8"],"award-info":[{"award-number":["FAPESP 2011\/03862-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Corticosteroids cause muscle atrophy by acting on proteasomal and lysosomal systems and by affecting pathways related to muscular trophysm, such as the IGF-1\/PI-3k\/Akt\/mTOR. Omega-3 fatty acid (n-3) has been used beneficially to attenuate muscle atrophy linked to sepsis and cachexia; however, its effect on dexamethasone-induced muscle atrophy has not been evaluated.<jats:italic>Objectives<\/jats:italic>. We evaluated whether n-3 supplementation could mitigate the development of dexamethasone-induced muscle atrophy.<jats:italic>Methods<\/jats:italic>. Two groups of<jats:italic>Wistar<\/jats:italic>rats were orally supplemented with n-3 or vehicle solution for 40 days. In the last 10 days, dexamethasone, or saline solution, was administrated establishing four groups: control, dexamethasone, n-3, and dexamethasone + n-3. The cross-sectional areas of muscle fibers, gene expression (<jats:italic>MyoD, Myogenin, MuRF-1<\/jats:italic>, and<jats:italic>Atrogin-1<\/jats:italic>), and protein expression (Akt, GSK3<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>\u03b2<\/mml:mi><\/mml:mrow><\/mml:math>, FOXO3a, and mTOR) were assessed.<jats:italic>Results<\/jats:italic>. Dexamethasone induced a significant loss in body and muscle weight, atrophy in type 2B fibers, and decreased expression of P-Akt, P-GSK3<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mi>\u03b2<\/mml:mi><\/mml:mrow><\/mml:math>, and P-FOXO3a. N-3 supplementation did not attenuate the negative effects of dexamethasone on skeletal muscle; instead, it caused atrophy in type 1, 2A, reduced the expression of<jats:italic>Myogenin<\/jats:italic>, and increased the expression of<jats:italic>Atrogin-1<\/jats:italic>.<jats:italic>Conclusion<\/jats:italic>. Food supplements containing n-3 are usually healthful, but they may potentiate some of the side effects of glucocorticoids.<\/jats:p>","DOI":"10.1155\/2014\/961438","type":"journal-article","created":{"date-parts":[[2014,5,25]],"date-time":"2014-05-25T21:11:39Z","timestamp":1401052299000},"page":"1-13","source":"Crossref","is-referenced-by-count":21,"title":["The Effects of Omega-3 Fatty Acid Supplementation on Dexamethasone-Induced Muscle Atrophy"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4015-9262","authenticated-orcid":true,"given":"Alan","family":"Fappi","sequence":"first","affiliation":[{"name":"Department of Neurology, University of S\u00e3o Paulo, No. 455, Dr. Arnaldo Avenue, 01246-903 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiago S.","family":"Godoy","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of S\u00e3o Paulo, No. 455, Dr. Arnaldo Avenue, 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