{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:24:13Z","timestamp":1760865853324,"version":"3.37.3"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Chinese National Programs for Fundamental Research and Development","award":["2010CB530202","2013JB04"],"award-info":[{"award-number":["2010CB530202","2013JB04"]}]},{"DOI":"10.13039\/501100005196","name":"Chinese Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["2010CB530202","2013JB04"],"award-info":[{"award-number":["2010CB530202","2013JB04"]}],"id":[{"id":"10.13039\/501100005196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Malate dehydrogenase (MDH) plays important metabolic roles in bacteria. In this study, the recombinant MDH protein (His-MDH) of<jats:italic>Brucella abortus<\/jats:italic>was purified and its ability to catalyze the conversion of oxaloacetate (OAA) to L-malate (hereon referred to as MDH activity) was analyzed. Michaelis Constant (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant=\"normal\">K<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>m<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>) and Maximum Reaction Velocity (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant=\"normal\">V<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>max<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>) of the reaction were determined to be<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mn>6.45<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mo>\u2212<\/mml:mo><mml:mn>3<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>M and 0.87\u2009mM\u2009L<jats:sup>\u22121\u2009<\/jats:sup>min<jats:sup>\u22121<\/jats:sup>, respectively.<jats:italic>In vitro<\/jats:italic>studies showed that His-MDH exhibited maximal MDH activity in pH 6.0 reaction buffer at 40\u00b0C. The enzymatic activity was 100%, 60%, and 40% inhibited by Cu<jats:sup>2+<\/jats:sup>, Zn<jats:sup>2+<\/jats:sup>, and Pb<jats:sup>2+<\/jats:sup>, respectively. In addition, six amino acids in the MDH were mutated to investigate their roles in the enzymatic activity. The results showed that the substitutions of amino acids Arg 89, Asp 149, Arg 152, His 176, or Thr 231 almost abolished the activity of His-MDH. The present study will help to understand MDH\u2019s roles in<jats:italic>B. abortus<\/jats:italic>metabolism.<\/jats:p>","DOI":"10.1155\/2014\/973751","type":"journal-article","created":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T17:19:39Z","timestamp":1399483179000},"page":"1-8","source":"Crossref","is-referenced-by-count":3,"title":["Enzymatic Activity Analysis and Catalytic Essential Residues Identification of<i>Brucella abortus<\/i>Malate Dehydrogenase"],"prefix":"10.1155","volume":"2014","author":[{"given":"Xiangan","family":"Han","sequence":"first","affiliation":[{"name":"Shanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongliang","family":"Tong","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, 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