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Twenty-one clinical strains of<jats:italic>Scedosporium<\/jats:italic>from China and two strains from Japan were reidentified by MLSA. The analysis included BT2, CAL, RPB, SOD, and ACT and the combination of the five loci.<jats:italic>Pseudallescheria boydii<\/jats:italic>complex (17 strains) and<jats:italic>S. apiospermum<\/jats:italic>(6 strains) were identified.<jats:italic>P. boydii<\/jats:italic>complex included four closely related subgroups:<jats:italic>P. boydii<\/jats:italic>(9 strains),<jats:italic>P. ellipsoidea<\/jats:italic>(6 strains),<jats:italic>P. fusoidea<\/jats:italic>(1 strain), and<jats:italic>P. angusta<\/jats:italic>(1 strain). There were no significant differences in MICs for neither VOR, POS, nor AMB over all the five species in study. 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