{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:18:41Z","timestamp":1790468321341,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T00:00:00Z","timestamp":1422316800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100007680","name":"Naval Medical Research Center","doi-asserted-by":"publisher","award":["6000.RAD1.J.A0310"],"award-info":[{"award-number":["6000.RAD1.J.A0310"]}],"id":[{"id":"10.13039\/100007680","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Bacteriology"],"published-print":{"date-parts":[[2015,1,27]]},"abstract":"<jats:p>Leptospirosis is a worldwide zoonosis caused by an infection with the pathogenic species of <jats:italic>Leptospira<\/jats:italic>. We have developed a loop-mediated isothermal amplification (LAMP) assay to detect the DNA of <jats:italic>Leptospira<\/jats:italic> spp. Six sets of primers targeting the gene of the subsurface protein, <jats:italic>lipL32<\/jats:italic>, were evaluated for their detection sensitivity. The best primer set detected less than 25 copies of <jats:italic>lipL32<\/jats:italic> per reaction of both plasmid DNA template and purified leptospiral genomic DNA. By combining primers targeting <jats:italic>lipL32<\/jats:italic> with the previously published primer set targeting <jats:italic>lipL41<\/jats:italic>, the sensitivity of the assay was improved to 12 copies of <jats:italic>L. interrogans<\/jats:italic>. The specificity of the LAMP assay was evaluated by using the genomic DNA from other clinically encountered bacterial species such as different strains of <jats:italic>Orientia tsutsugamushi<\/jats:italic>, <jats:italic>Rickettsia typhi<\/jats:italic>, <jats:italic>Rickettsia conorii<\/jats:italic>, <jats:italic>Rickettsia rickettsii<\/jats:italic>, <jats:italic>Coxiella burnetii<\/jats:italic>, and <jats:italic>Bartonella bacilliformis<\/jats:italic>. These genomic DNA samples were all negative in our LAMP assay. The sensitivity of the LAMP assay was very similar to that of quantitative real time PCR. Several detection methods for the amplified product of LAMP assay were performed to demonstrate the simplicity of the assay. In summary, our results have suggested that this assay is rapid, robust, and easy to perform and has the potential to be used in endemic locations.<\/jats:p>","DOI":"10.1155\/2015\/147173","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T21:01:43Z","timestamp":1422392503000},"page":"1-6","source":"Crossref","is-referenced-by-count":33,"title":["Highly Sensitive Loop-Mediated Isothermal Amplification for the Detection of <i>Leptospira<\/i>"],"prefix":"10.1155","volume":"2015","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6524-1292","authenticated-orcid":true,"given":"Hua-Wei","family":"Chen","sequence":"first","affiliation":[{"name":"Naval Medical Research Center, Silver Spring, MD 20910, USA"},{"name":"Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Weissenberger","sequence":"additional","affiliation":[{"name":"Naval Medical 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