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Matrine, the active ingredient of<jats:italic>Sophora<\/jats:italic>, had been used for clinical treatment of acute\/chronic liver disease. However, its potency was low. We prepared a high potency and low toxicity matrine derivate, WM130 (C<jats:sub>30<\/jats:sub>N<jats:sub>4<\/jats:sub>H<jats:sub>40<\/jats:sub>SO<jats:sub>5<\/jats:sub>F), which exhibited better pharmacological activities on antihepatic fibrosis. This study demonstrated that WM130 results in a decreased proliferative activity of HSC-T6 cells, with the half inhibitory concentration (IC<jats:sub>50<\/jats:sub>) of 68\u2009<jats:italic>\u03bc<\/jats:italic>M. WM130 can inhibit the migration and induce apoptosis in HSC-T6 cells at both concentrations of 68\u2009<jats:italic>\u03bc<\/jats:italic>M (IC<jats:sub>50<\/jats:sub>) and 34\u2009<jats:italic>\u03bc<\/jats:italic>M (half IC<jats:sub>50<\/jats:sub>). The expression of<jats:italic>\u03b1<\/jats:italic>-SMA, Collagen I, Collagen III, and TGF-<jats:italic>\u03b2<\/jats:italic>1 could be downregulated, and the protein phosphorylation levels of EGFR, AKT, ERK, Smad, and Raf (p-EGFR, p-AKT, p-ERK, p-Smad, and p-Raf) were also decreased by WM130. On the DMN-induced rat liver fibrosis model, WM130 can effectively reduce the TGF-<jats:italic>\u03b2<\/jats:italic>1, AKT,<jats:italic>\u03b1<\/jats:italic>-SMA, and p-ERK levels, decrease the extracellular matrix (ECM) formation, and inhibit rat liver fibrosis progression. In conclusion, this study demonstrated that WM130 can significantly inhibit the activation of HSC-T6 cells and block the rat liver fibrosis progression by inducing apoptosis, suppressing the deposition of ECM, and inhibiting TGF-<jats:italic>\u03b2<\/jats:italic>\/Smad and Ras\/ERK pathways.<\/jats:p>","DOI":"10.1155\/2015\/203978","type":"journal-article","created":{"date-parts":[[2015,6,18]],"date-time":"2015-06-18T17:01:22Z","timestamp":1434646882000},"page":"1-13","source":"Crossref","is-referenced-by-count":11,"title":["A Novel Matrine Derivative WM130 Inhibits Activation of Hepatic Stellate Cells and Attenuates Dimethylnitrosamine-Induced Liver Fibrosis in Rats"],"prefix":"10.1155","volume":"2015","author":[{"given":"Yang","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Molecular Oncology, Eastern Hepatobiliary Surgery Hospital and National Center of Liver Cancer, Second Military Medical University, Shanghai 200438, 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