{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:27Z","timestamp":1740169767839,"version":"3.37.3"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31172316","1210RJIA006"],"award-info":[{"award-number":["31172316","1210RJIA006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003999","name":"Science Fund for Creative Research Groups","doi-asserted-by":"publisher","award":["31172316","1210RJIA006"],"award-info":[{"award-number":["31172316","1210RJIA006"]}],"id":[{"id":"10.13039\/501100003999","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic>with worldwide distribution has received substantial medical and scientific attentions as it causes serious clinical and veterinary problems especially for pregnant women and immunocompromised patients. Heat shock protein 40 (HSP40) plays a variety of essential roles in the pathogenesis of this protozoan parasite. In order to detail the genetic diversity of HSP40 gene, 16<jats:italic>T. gondii<\/jats:italic>strains from different hosts and geographical locations were used in this study. Our results showed that HSP40 sequence of the examined strains was between 6621\u2009bp and 6644\u2009bp in length, and their A+T content was from 48.54% to 48.80%. Furthermore, sequence analysis presented 195 nucleotide mutation positions (0.12%\u20131.14%) including 29 positions in CDS (0.02%\u20130.12%) compared with<jats:italic>T. gondii<\/jats:italic>ME49 strain (ToxoDB: TGME49_265310). Phylogenetic assay revealed that<jats:italic>T. gondii<\/jats:italic>strains representing three classical genotypes (Types I, II, and III) were completely separated into different clusters by maximum parsimony (MP) method, but Type II and ToxoDB#9 strains were grouped into the same cluster. These results suggested that HSP40 gene is not a suitable marker for<jats:italic>T. gondii<\/jats:italic>population genetic research, though three classical genotypes of<jats:italic>T. gondii<\/jats:italic>could be differentiated by restriction enzymes<jats:italic>Msc<\/jats:italic>I and<jats:italic>Ear<\/jats:italic>I existing in amplicon C.<\/jats:p>","DOI":"10.1155\/2015\/209792","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T16:01:30Z","timestamp":1446566490000},"page":"1-6","source":"Crossref","is-referenced-by-count":1,"title":["Sequence Variation in HSP40 Gene among 16<i>Toxoplasma gondii<\/i>Isolates from Different Hosts and Geographical Locations"],"prefix":"10.1155","volume":"2015","author":[{"given":"Zhong-Yuan","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese 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