{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:56:54Z","timestamp":1791316614614,"version":"4.3.1"},"reference-count":55,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>The<jats:italic>Sporothrix<\/jats:italic>complex members cause sporotrichosis, a subcutaneous mycosis with a wide spectrum of clinical manifestations. Several specific phenotypic characteristics are associated with virulence in many fungi, but studies in this field involving the<jats:italic>Sporothrix<\/jats:italic>complex species are scarce. Melanization, thermotolerance, and production of proteases, catalase, and urease were investigated in 61<jats:italic>S. brasiliensis<\/jats:italic>, one<jats:italic>S. globosa<\/jats:italic>, and 10<jats:italic>S. schenckii<\/jats:italic>strains. The<jats:italic>S. brasiliensis<\/jats:italic>strains showed a higher expression of melanin and urease compared with<jats:italic>S. schenckii<\/jats:italic>. These two species, however, presented similar thermotolerances. Our<jats:italic>S. globosa<\/jats:italic>strain had low expression of all studied virulence factors. The relationship between these phenotypes and clinical aspects of sporotrichosis was also evaluated. Strains isolated from patients with spontaneous regression of infection were heavily melanized and produced high urease levels. Melanin was also related to dissemination of internal organs and protease production was associated with HIV-coinfection. A murine sporotrichosis model showed that a<jats:italic>S. brasiliensis<\/jats:italic>strain with high expression of virulence factors was able to disseminate and yield a high fungal burden in comparison with a control<jats:italic>S. schenckii<\/jats:italic>strain. Our results show that virulence-related phenotypes are variably expressed within the<jats:italic>Sporothrix<\/jats:italic>complex species and might be involved in clinical aspects of sporotrichosis.<\/jats:p>","DOI":"10.1155\/2015\/212308","type":"journal-article","created":{"date-parts":[[2015,4,19]],"date-time":"2015-04-19T21:02:26Z","timestamp":1429477346000},"page":"1-10","source":"Crossref","is-referenced-by-count":79,"title":["Phenotypic Characteristics Associated with Virulence of Clinical Isolates from the<i>Sporothrix<\/i>Complex"],"prefix":"10.1155","volume":"2015","author":[{"given":"Rodrigo","family":"Almeida-Paes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Avenida Brasil 4365, 21045-900 Manguinhos, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00e3 Cardoso","family":"de 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