{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:41:36Z","timestamp":1758404496990},"reference-count":22,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Silesia in Katowice","award":["KNW-1-006\/N\/4\/0","KNW-1-024\/N\/5\/0"],"award-info":[{"award-number":["KNW-1-006\/N\/4\/0","KNW-1-024\/N\/5\/0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Mesterolone is a synthetic androgenic steroid indicating a weak anabolic activity. A new, simple in use, and economical TLC-densitometric method in normal phase system (NP-TLC) has been developed and validated for the identification and quantitative determination of mesterolone in bulk drug and in tablet formulation. NP-TLC analysis was performed on aluminium plates precoated with silica gel 60F<jats:sub>254<\/jats:sub>as the stationary phase using chloroform-acetone (40\u2009:\u200910, v\/v) as mobile phase. Densitometric analysis was carried out at<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>\u03bb<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">745<\/mml:mn><\/mml:math>\u2009nm after staining with phosphomolybdic acid. These conditions were found to give visible (dark blue) spot and sharp peak, respectively, for mesterolone at<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:msub><mml:mrow><mml:mi>R<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi>F<\/mml:mi><\/mml:mrow><\/mml:msub><mml:mo>\u2009<\/mml:mo><mml:mo>\u2009<\/mml:mo><mml:mn fontstyle=\"italic\">0.75<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.02<\/mml:mn><\/mml:math>and enabled satisfactory separation of mesterolone from its related substance (potential impurity). The proposed NP-TLC-densitometric method was validated for specificity, linearity, precision, accuracy, robustness, and sensitivity according to ICH guideline and other validation requirements. The limit of detection (LOD) and limit of quantification (LOQ) were 61.0\u2009ng\u00b7spot<jats:sup>\u22121<\/jats:sup>and 184.0\u2009ng\u00b7spot<jats:sup>\u22121<\/jats:sup>, respectively. The percent content of mesterolone in marketed tablet formulation was found to be 99.40% of label claim. The developed TLC-densitometric method can be successfully used in quality control of mesterolone in bulk material and also tablet formulation.<\/jats:p>","DOI":"10.1155\/2015\/230104","type":"journal-article","created":{"date-parts":[[2015,12,31]],"date-time":"2015-12-31T21:03:38Z","timestamp":1451595818000},"page":"1-8","source":"Crossref","is-referenced-by-count":5,"title":["A Validated TLC-Densitometric Method for the Determination of Mesterolone in Bulk Material and in Tablets"],"prefix":"10.1155","volume":"2015","author":[{"given":"Ma\u0142gorzata","family":"Do\u0142owy","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, School of Pharmacy and the Division of Laboratory Medicine, Medical University of Silesia in Katowice, 4 Jagiello\u0144ska, 41-200 Sosnowiec, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina","family":"Pyka-Paj\u0105k","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, School of Pharmacy and the Division of Laboratory Medicine, Medical University of Silesia in Katowice, 4 Jagiello\u0144ska, 41-200 Sosnowiec, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"Filip","sequence":"additional","affiliation":[{"name":"R&D Analytical Chemistry Department, Pharmaceutical Research Institute, 8 Rydygiera, 01-793 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Zagrodzka","sequence":"additional","affiliation":[{"name":"R&D Analytical Chemistry Department, Pharmaceutical Research Institute, 8 Rydygiera, 01-793 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"issue":"3","key":"1","first-page":"17","volume":"2","year":"2013","journal-title":"International Journal of Pharmaceutical Science Invention"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.2165\/00007256-200434080-00003"},{"key":"3","first-page":"411","volume":"195","year":"2010","journal-title":"Handbook of Experimental Pharmacology"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.2174\/1570159x13666141210224756"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(89)80210-9"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3508"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/35.1.38"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(92)90146-a"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-4319-y"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(92)90145-9"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.5012\/bkcs.2009.30.7.1489"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/BF02704760"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1367"},{"issue":"3","key":"14","first-page":"301","volume":"9","year":"2010","journal-title":"Tropical Journal of Pharmaceutical Research"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1186\/1752-153x-6-54"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2014.907113"},{"issue":"6","key":"17","first-page":"922","volume":"71","year":"2014","journal-title":"Acta Poloniae Pharmaceutica: Drug Research"},{"key":"18","series-title":"Q2(R1)","year":"2005"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1556\/jpc.23.2010.3.1"},{"key":"20","first-page":"349","volume":"8","year":"1999","journal-title":"Journal of Planar Chromatography\u2014Modern TLC"},{"key":"21","first-page":"225","volume-title":"Validation of analytical procedures","year":"2007"},{"key":"22","year":"1999"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/230104.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/230104.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/230104.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T15:47:05Z","timestamp":1471535225000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2015\/230104\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":22,"alternative-id":["230104","230104"],"URL":"https:\/\/doi.org\/10.1155\/2015\/230104","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}