{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:39:37Z","timestamp":1761597577244,"version":"3.40.5"},"reference-count":49,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Inflammation"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Aim and Methods<\/jats:italic>. In this study, we evaluated NOS and arginase activities and their regulation during Beh\u00e7et disease, a systemic chronic inflammatory disorder with uncertain etiology. The peripheral blood mononuclear cells of 36 patients and 15 control samples (PBMC) were cultured in either RPMI 1640, MEM, or DMEM complemented with 10% of FBS and antibiotics. Cultures were performed with or without the control or patients plasma. Subsequent treatment contained anticytokines (IL-6, TGF-<jats:italic>\u03b2<\/jats:italic>), a mitogenic effector (PHA), or NOS modulators (L-NMMA, BH4). Culture supernatants were harvested after 24\u2009h of incubation. NO and urea measurements were, respectively, performed by modified Griess and Berthelot methods.<jats:italic>Results<\/jats:italic>. Higher urea levels were found in patients\u2019 plasma compared to the control\u2019s (<jats:italic>P<\/jats:italic>&lt; 0.05). NOS modulators induced inverted production profiles for NO and urea (<jats:italic>P<\/jats:italic>&lt; 0.05). Their results differed depending on the clinical findings (<jats:italic>P<\/jats:italic>&lt; 0.05). It was also found that cytokine neutralization induced different response profiles in patients as opposed to control cultures (<jats:italic>P<\/jats:italic>&lt; 0.05).<jats:italic>Conclusion<\/jats:italic>. Our results suggest that arginases can compete with NOS2 for L-arginine during Beh\u00e7et disease. Both enzymes are regulated by environmental cytokines and substrate availability. Furthermore, it seems that NOS\/arginase balance is dependent on clinical expression.<\/jats:p>","DOI":"10.1155\/2015\/241738","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T21:02:08Z","timestamp":1422392528000},"page":"1-9","source":"Crossref","is-referenced-by-count":9,"title":["Cytokines Modulate the \u201cImmune-Metabolism\u201d Interactions during Beh\u00e7et Disease: Effect on Arginine Metabolism"],"prefix":"10.1155","volume":"2015","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3641-4291","authenticated-orcid":true,"given":"Houda","family":"Belguendouz","sequence":"first","affiliation":[{"name":"USTHB, \u201cCytokines and NO Synthases\u201d Team, LBCM, FSB, BP 32 El Alia, Bab Ezzouar, 16111 Algiers, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karima","family":"Lahmar-Belguendouz","sequence":"additional","affiliation":[{"name":"USTHB, \u201cCytokines and NO Synthases\u201d Team, LBCM, FSB, BP 32 El Alia, Bab 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