{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:55:50Z","timestamp":1785308150272,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Medical University of Bialystok, Poland"},{"name":"Leading National Research Center in Bialystok"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>An increase in the antibiotic resistance among members of the<jats:italic>Enterobacteriaceae<\/jats:italic>family has been observed worldwide. Multidrug-resistant Gram-negative rods are increasingly reported. The treatment of infections caused by<jats:italic>Escherichia coli<\/jats:italic>and other<jats:italic>Enterobacteriaceae<\/jats:italic>has become an important clinical problem associated with reduced therapeutic possibilities. Antimicrobial carbapenems are considered the last line of defense against multidrug-resistant Gram-negative bacteria. Unfortunately, an increase of carbapenem resistance due to the production of<jats:italic>Klebsiella pneumoniae<\/jats:italic>carbapenemase (KPC) enzymes has been observed. In this study we describe the ability of<jats:italic>E. coli<\/jats:italic>to produce carbapenemase enzymes based on the results of the combination disc assay with boronic acid performed according to guidelines established by the European Community on Antimicrobial Susceptibility Testing (EUCAST) and the biochemical Carba NP test. Moreover, we evaluated the presence of genes responsible for the production of carbapenemases (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>KPC<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>VIM<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>IMP<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>OXA-48<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>) and genes encoding other<jats:italic>\u03b2<\/jats:italic>-lactamases (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>SHV<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>TEM<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>CTX-M<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>) among<jats:italic>E. coli<\/jats:italic>isolate. The tested isolate of<jats:italic>E. coli<\/jats:italic>that possessed the<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M8\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>KPC-3<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M9\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>TEM-34<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>genes was identified. The tested strain exhibited susceptibility to colistin (0.38\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) and tigecycline (1\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL). This is the first detection of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M10\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>KPC-3<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>in an<jats:italic>E. coli<\/jats:italic>ST479 in Poland.<\/jats:p>","DOI":"10.1155\/2015\/256028","type":"journal-article","created":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T02:26:53Z","timestamp":1438655213000},"page":"1-3","source":"Crossref","is-referenced-by-count":9,"title":["First Report of<i>Klebsiella pneumoniae<\/i>-Carbapenemase-3-Producing<i>Escherichia coli<\/i>ST479 in Poland"],"prefix":"10.1155","volume":"2015","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7790-0328","authenticated-orcid":true,"given":"Dominika","family":"Ojdana","sequence":"first","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawe\u0142","family":"Sacha","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Olsza\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, University Hospital of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3097-4581","authenticated-orcid":true,"given":"Piotr","family":"Majewski","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Wieczorek","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jadwiga","family":"Jaworowska","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, University Hospital of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sie\u0144ko","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Jurczak","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, University Hospital of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u017cbieta","family":"Tryniszewska","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1086\/432803"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70054-4"},{"key":"3","series-title":"Version 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