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Clone 11-25 is a murine hybridoma secreting monoclonal antibody (mAb) against human MSLN. In this study, we applied the 11-25 mAb to<jats:italic>in vivo<\/jats:italic>imaging to detect MSLN-expressing tumors. In<jats:italic>in vitro<\/jats:italic>and<jats:italic>ex vivo<\/jats:italic>immunochemical studies, we demonstrated specificity of 11-25 mAb to membranous MSLN expressed on several pancreatic cancer cells. We showed the accumulation of Alexa Fluor 750-labeled 11-25 mAb in MSLN-expressing tumor xenografts in athymic nude mice. Then, 11-25 mAb was labeled with<jats:sup>64<\/jats:sup>Cu via a chelating agent DOTA and was used in both<jats:italic>in vitro<\/jats:italic>cell binding assay and<jats:italic>in vivo<\/jats:italic>positron emission tomography (PET) imaging in the tumor-bearing mice. We confirmed that<jats:sup>64<\/jats:sup>Cu-labeled 11-25 mAb highly accumulated in MSLN-expressing tumors as compared to MSLN-negative ones. The<jats:sup>64<\/jats:sup>Cu-labeled 11-25 mAb is potentially useful as a PET probe capable of being used for wide range of tumors, rather than<jats:sup>18<\/jats:sup>F-FDG that occasionally provides nonspecific accumulation into the inflammatory lesions.<\/jats:p>","DOI":"10.1155\/2015\/268172","type":"journal-article","created":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T19:26:07Z","timestamp":1427311567000},"page":"1-15","source":"Crossref","is-referenced-by-count":40,"title":["A Novel PET Imaging Using<sup><b>64<\/b><\/sup>Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells"],"prefix":"10.1155","volume":"2015","author":[{"given":"Kazuko","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8558, Japan"},{"name":"Department of Urology, Okayama University Graduate School of Medicine, Dentistry, and 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