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Malignant mesothelioma (MM) is an aggressive tumor of the serosal membranes, mostly the pleura. It is related to asbestos exposure and has a poor prognosis. MM has a long latency period, and incidence is predicted to remain stable or increase until 2020. Currently, no biomarkers for a specific targeted therapy are available. Previously, we observed that expression of aquaporin 1 (AQP1) was an indicator of prognosis in two independent cohorts. Here we determine whether AQP1 inhibition has therapeutic potential in the treatment of MM.<jats:italic>Methods<\/jats:italic>. Functional studies were performed with H226 cells and primary MM cells harvested from pleural effusions. AQP1 expression and mesothelial phenotype was determined by immunohistochemistry. AQP1 function was inhibited by a pharmacological blocker (AqB050) or AQP1-specific siRNA. Cell proliferation, migration, and anchorage-independent cell growth were assessed. A nude mouse heterotopic xenograft model of MM was utilised for the<jats:italic>in vivo<\/jats:italic>studies.<jats:italic>Results<\/jats:italic>. Inhibition of AQP1 significantly decreases cell proliferation, metastatic potential, and motility without inducing nonspecific cytotoxicity or increasing apoptosis.<jats:italic>In vivo<\/jats:italic>blockade of AQP1 had no biologically significant effect on growth of established tumours.<jats:italic>Conclusions<\/jats:italic>. Targeted blockade of AQP1 restricts MM growth and migration<jats:italic>in vitro<\/jats:italic>. Further work is warranted to fully evaluate treatment potential<jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2015\/286719","type":"journal-article","created":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T16:00:33Z","timestamp":1425484833000},"page":"1-9","source":"Crossref","is-referenced-by-count":22,"title":["Blockade of Aquaporin 1 Inhibits Proliferation, Motility, and Metastatic Potential of Mesothelioma<i>In Vitro<\/i>but not in an<i>In Vivo<\/i>Model"],"prefix":"10.1155","volume":"2015","author":[{"given":"Sonja","family":"Klebe","sequence":"first","affiliation":[{"name":"Department of Anatomical Pathology, Flinders University, Adelaide, Bedford, SA 5042, Australia"},{"name":"SA Pathology, Department of Surgical Pathology, Flinders Medical Centre, Adelaide, Bedford, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Griggs","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology, 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